Proteintech, 16400-1-AP, UBQLNL Polyclonal antibody

Size: 20ul / 150ul The UBQLNL (16400-1-AP) by Proteintech is a Polyclonal antibody targeting UBQLNL in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human, mouse samples 16400-1-AP targets UBQLNL in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: human brain tissue, Jurkat cells Positive IP detected in: Jurkat cells Positive IHC detected in: human colon tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: A431 cells Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100 Background Information UBQLNL also named as Ubiquilin like protein, encodes a 475 amino acid protein. The specific function of it is still non-known. UBQLNL may have an association with type 2 diabetes, but the mechanism is supposed be studied in the further research. The subcellular localization of UBQLNL is nucleus. The expression of it is detected in some tissues, such as brain ,testis, heart and liver. Specification Tested Reactivity: human, mouse Cited Reactivity: mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag9496 Product name: Recombinant human UBQLNL protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 132-475 aa of BC012183 Sequence: SSRVHQPTGMNQAPVELAHFVGSDAPKVHTQNLEVSHPECKAQMLENPSIQRLLSNMEFMWQFISEHLDTQQLMQQNPEVSRLLLDNSEILLQTLELARNLAMIQEIMQIQQPSQNLEYPLNPQPYLGLETMPGGNNALGQNYVDINDQMLNSMQDPFGGNPFTALLAGQVLEQVQSSPPPPPPSQEQQDQLTQHPATRVIYNSSGGFSSNTSANDTLNKVNHTSKANTAMISTKGQSHICATRQPAGIPALPSIELTQQLQEEYKDATVSLSSSRQTLKGDLQLSDEQSSSQITGGMMQLLMNNPYLAAQIMLFTSMPQLSEEWRQQLPTFLQQTQISDLLSA Predict reactive species Full Name: ubiquilin-like Calculated Molecular Weight: 475 aa, 53 kDa Observed Molecular Weight: 70 kDa GenBank Accession Number: BC012183 Gene Symbol: UBQLNL Gene ID (NCBI): 143630 RRID: AB_2878253 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q8IYU4 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.