Proteintech, 16417-1-AP, BCAT2 Polyclonal antibody

Size: 20ul / 150ul The BCAT2 (16417-1-AP) by Proteintech is a Polyclonal antibody targeting BCAT2 in WB, IHC, IF/ICC, FC (Intra), ELISA applications with reactivity to human, mouse samples 16417-1-AP targets BCAT2 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: Jurkat cells, L02 cells, Raji cells, RAW 264.7 cells, MCF-7 cells, MDA-MB-231 cells Positive IHC detected in: human skin tissue, human placenta tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: C2C12 cells Positive FC (Intra) detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:6000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension Background Information Branched-chain amino acid transaminase 2 (BCAT2) is an important enzyme in branched-chain amino acids (BCAAs) catabolism that reversibly catalyzes the initial step of BCAA degradation to branched-chain acyl-CoA. In humans, BCAT2 encodes a mitochondrial protein that is ubiquitously expressed in all organs (except hepatocytes) (PMID:32467562). Moreover, other reports showed that overexpressed BCAT2 in pancreatic ductal adenocarcinoma (PDAC) cells enhanced cell proliferation and survival in vitro and in vivo (PMID:35663242). Specification Tested Reactivity: human, mouse Cited Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag9573 Product name: Recombinant human BCAT2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 41-392 aa of BC001900 Sequence: MTQKPHKKPGPGEPLVFGKTFTDHMLMVEWNDKGWGQPRIQPFQNLTLHPASSSLHYSLQLFEGMKAFKGKDQQVRLFRPWLNMDRMLRSAMRLCLPSFDKLELLECIRRLIEVDKDWVPDAAGTSLYVRPVLIGNEPSLGVSQPTRALLFVILCPVGAYFPGGSVTPVSLLADPAFIRAWVGGVGNYKLGGNYGPTVLVQQEALKRGCEQVLWLYGPDHQLTEVGTMNIFVYWTHEDGVLELVTPPLNGVILPGVVRQSLLDMAQTWGEFRVVERTITMKQLLRALEEGRVREVFGSGTACQVCPVHRILYKDRNLHIPTMENGPELILRFQKELKEIQYGIRAHEWMFPV Predict reactive species Full Name: branched chain aminotransferase 2, mitochondrial Calculated Molecular Weight: 44 kDa Observed Molecular Weight: 38-42 kDa GenBank Accession Number: BC001900 Gene Symbol: BCAT2 Gene ID (NCBI): 587 RRID: AB_10792411 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: O15382 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.