Proteintech, 16451-1-AP, MCM8 Polyclonal antibody

Size: 20ul / 150ul The MCM8 (16451-1-AP) by Proteintech is a Polyclonal antibody targeting MCM8 in WB, IP, ELISA applications with reactivity to human, mouse, rat samples 16451-1-AP targets MCM8 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HEK-293T cells, NIH/3T3 cells Positive IP detected in: NIH/3T3 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:5000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Background Information MCM8, also named as Minichromosome maintenance 8, is a 840 amino acid protein, which belongs to the MCM family. MCM8 is expressed highly in placenta, lung and pancreas and various tumors with highest levels in colon and lung cancers. MCM8 is a component of the MCM8-MCM9 complex, which involved in homologous recombination repair following DNA interstrand cross-links and plays a key role during gametogenesis. MCM8 exists some isoforms with MV 94 kDa and 70 kDa. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag8696 Product name: Recombinant human MCM8 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 380-735 aa of BC008830 Sequence: SGLTVTLSKDSSSGDFALEAGALVLGDQGICGIDEFDKMGNQHQALLEAMEQQSISLAKAGVVCSLPARTSIIAAANPVGGHYNKAKTVSENLKMGSALLSRFDLVFILLDTPNEHHDHLLSEHVIAIRAGKQRTISSATVARMNSQDSNTSVLEVVSEKPLSERLKVVPGETIDPIPHQLLRKYIGYARQYVYPRLSTEAARVLQDFYLELRKQSQRLNSSPITTRQLESLIRLTEARARLELREEATKEDAEDIVEIMKYSMLGTYSDEFGNLDFERSQHGSGMSNRSTAKRFISALNNVAERTYNNIFQFHQLRQIAKELNIQVADFENFIGSLNDQGYLLKKGPKVYQLQTM Predict reactive species Full Name: minichromosome maintenance complex component 8 Calculated Molecular Weight: 880 aa, 98 kDa Observed Molecular Weight: 90-95 kDa GenBank Accession Number: BC008830 Gene Symbol: MCM8 Gene ID (NCBI): 84515 RRID: AB_2142833 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9UJA3 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.