Proteintech, 16600-1-AP, SPTLC3 Polyclonal antibody

Size: 20ul / 150ul The SPTLC3 (16600-1-AP) by Proteintech is a Polyclonal antibody targeting SPTLC3 in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse samples 16600-1-AP targets SPTLC3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: HepG2 cells, mouse liver tissue Positive IHC detected in: human ovary cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Immunohistochemistry (IHC): IHC : 1:250-1:1000 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information Serine-palmitoyltransferase (SPT) is a pyridoxal 5‐phosphate (PLP)-dependent α‐oxoaminotransferase that consists of three core subunits-SPTLC1, SPTLC2, and SPTLC3-that share a mutual homology. The amino acid sequences between SPTLC1 and SPTLC2 (or SPTLC3) show a mutual similarity of about 20%, whereas the sequences for SPTLC2 and SPTLC3 show a significantly higher identity of 68% (with 84% similarity) on the amino acid level. SPTLC3 is not or only little expressed in tissues that are functionally connected to erythropoiesis or immune response like peripheral blood cells, bone marrow, and spleen. Instead, the lack of SPTLC3 expression seems to be compensated by increased expression of SPTLC2 in these tissues. (PMID: 17331073, PMID: 17023427) Specification Tested Reactivity: human, mouse Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag9760 Product name: Recombinant human SPTLC3 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-175 aa of BC020656 Sequence: MANPGGGAVCNGKLHNHKKQSNGSQSRNCTKNGIVKEAQQNGKPHFYDKLIVESFEEAPLHVMVFTYMGYGIGTLFGYLRDFLRNWGIEKCNAAVERKEQKVRMRTSLDLCQCLLLSKVFSEVVMQVQILESMRCSGTIQGKFHSSPPAKPHYPWAYGPVFTNISWATTICHIPN Predict reactive species Full Name: serine palmitoyltransferase, long chain base subunit 3 Calculated Molecular Weight: 175 aa, 20 kDa Observed Molecular Weight: 62 kDa GenBank Accession Number: BC020656 Gene Symbol: SPTLC3 Gene ID (NCBI): 55304 RRID: AB_3085500 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9NUV7 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.