Proteintech, 16605-1-AP, OTUD7B Polyclonal antibody

Size: 20ul / 150ul The OTUD7B (16605-1-AP) by Proteintech is a Polyclonal antibody targeting OTUD7B in WB, IP, IHC, ELISA applications with reactivity to human, mouse, rat samples 16605-1-AP targets OTUD7B in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: Jurkat cells Positive IP detected in: Jurkat cells Positive IHC detected in: human kidney tissue, human heart tissue, mouse kidney tissue, mouse heart tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information OUTD7B is a member of the A20 family of the deubiquitinating cysteine proteases, which has deubiquitinating activity that is directed towards 'Lys-48' or 'Lys-63'-linked polyubiquitin chains. It plays a key role in the negative feedback regulation of NF-κB signaling in response to multiple stimuli and has been described as central gatekeeper in inflammation and immunity. The calculated molecular weight of OTUD7B is 93 kDa, but the modified OTUD7B is about 100 kDa. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag9788 Product name: Recombinant human OTUD7B protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-355 aa of BC020622 Sequence: MTLDMDAVLSDFVRSTGAEPGLARDLLEGKNWDVNAALSDFEQLRQVHAGNLPPSFSEGSGGSRTPEKGFSDREPTRPPRPILQRQDDIVQEKRLSRGISHASSSIVSLARSHVSSNGGGGGSNEHPLEMPICAFQLPDLTVYNEDFRSFIERDLIEQSMLVALEQAGRLNWWVSVDPTSQRLLPLATTGDGNCLLHAASLGMWGFHDRDLMLRKALYALMEKGVEKEALKRRWRWQQTQQNEESGLVYTEDEWQKEWNELIKLASSEPRMHLGTNGANCGGVESSEEPVYESLEEFHVFVLAHVLRRPIVVVADTMLRDSGGEAFAPIPFGGIYLPLEVPASQCHRSPLVLAYD Predict reactive species Full Name: OTU domain containing 7B Calculated Molecular Weight: 427aa,48 kDa; 843aa,93 kDa Observed Molecular Weight: 100 kDa GenBank Accession Number: BC020622 Gene Symbol: OTUD7B Gene ID (NCBI): 56957 RRID: AB_2157149 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q6GQQ9 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.