Proteintech, 16659-1-AP, DNase I Polyclonal antibody

Size: 20ul / 150ul The DNase I (16659-1-AP) by Proteintech is a Polyclonal antibody targeting DNase I in WB, ELISA applications with reactivity to human samples 16659-1-AP targets DNase I in WB, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: human urine sample Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Background Information Deoxyribonuclease I (DNase I), also known as DNL1 and DRNI, belongs to the DNase I family. DNase I is a DNA-digestive glycoprotein that cleaves both single- and double stranded DNA through hydrolysis of the phosphodiester bond. In humans, the DNase I enzyme is primarily secreted by the pancreas, parotid, and pituitary glands into the digestive system and bloodstream. Since DNase I is ubiquitously expressed in human tissues, it is thought to play a similar anti-autoimmune role in other organs as well (PMID: 21806320). The calculated molecular weight of DNase I is 31 kDa. Sometimes higher molecular weight around 40 kDa can also be observed, which is caused by glycosylation (PMID: 34329318). Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag10117 Product name: Recombinant human DNASE1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-282 aa of BC029437 Sequence: MRGMKLLGALLALAALLQGAVSLKIAAFNIQTFGETKMSNATLVSYIVQILSRYDIALVQEVRDSHLTAVGKLLDNLNQDAPDTYHYVVSEPLGRNSYKERYLFVYRPDQVSAVDSYYYDDGCEPCGNDTFNREPAIVRFFSRFTEVREFAIVPLHAAPGDAVAEIDALYDVYLDVQEKWGLEDVMLMGDFNAGCSYVRPSQWSSIRLWTSPTFQWLIPDSADTTATPTHCAYDRIVVAGMLLRGAVVPDSALPFNFQAAYGLSDQLAQAISDHYPVEVMLK Predict reactive species Full Name: deoxyribonuclease I Calculated Molecular Weight: 282 aa, 31 kDa Observed Molecular Weight: 31-40 kDa GenBank Accession Number: BC029437 Gene Symbol: DNASE1 Gene ID (NCBI): 1773 RRID: AB_3669253 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P24855 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.