Proteintech, 16741-1-AP, TBX3 Polyclonal antibody

Size: 20ul / 150ul The TBX3 (16741-1-AP) by Proteintech is a Polyclonal antibody targeting TBX3 in WB, IHC, IP, ELISA applications with reactivity to human samples 16741-1-AP targets TBX3 in WB, IHC, IP, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HepG2 cells Positive IP detected in: human placenta tissue Positive IHC detected in: human thyroid cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information TBX3, also named as T-box protein 3, is a 743 amino acid protein, which contains 1 T-box DNA-binding domain. TBX3 may localize in the nucleus and is widely expressed in various tissues. TBX3 as a transcriptional repressor is involved in developmental processes and probably plays a role in limb pattern formation. TBX3 plays an important role on osteogenic differentiation and proliferation of human mesenchymal stem cell derived from adipose tissue. Specification Tested Reactivity: human Cited Reactivity: human, marmoset Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag9955 Product name: Recombinant human TBX3 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 397-743 aa of BC025258 Sequence: TTTSEEPCRDKGSPAVKAHLFAAERPRDSGRLDKASPDSRHSPATISSSTRGLGAEERRSPVREGTAPAKVEEARALPGKEAFAPLTVQTDAAAAHLAQGPLPGLGFAPGLAGQQFFNGHPLFLHPSQFAMGGAFSSMAAAGMGPLLATVSGASTGVSGLDSTAMASAAAAQGLSGASAATLPFHLQQHVLASQGLAMSPFGSLFPYPYTYMAAAAAASSAAASSSVHRHPFLNLNTMRPRLRYSPYSIPVPVPDGSSLLTTALPSMAAAAGPLDGKVAALAASPASVAVDSGSELNSRSSTLSSSSMSLSPKLCAEKEAATSELQSIQRLVSGLEAKPDRSRSASP Predict reactive species Full Name: T-box 3 Calculated Molecular Weight: 79 kDa Observed Molecular Weight: 79 kDa GenBank Accession Number: BC025258 Gene Symbol: TBX3 Gene ID (NCBI): 6926 RRID: AB_2200696 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: O15119 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.