Proteintech, 16743-1-AP, PHKG1 Polyclonal antibody

Size: 20ul / 150ul The PHKG1 (16743-1-AP) by Proteintech is a Polyclonal antibody targeting PHKG1 in WB, IP, IHC, ELISA applications with reactivity to human, mouse, rat samples 16743-1-AP targets PHKG1 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: mouse skeletal muscle tissue, rat skeletal muscle tissue Positive IP detected in: mouse skeletal muscle tissue Positive IHC detected in: mouse skeletal muscle tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:1000-1:5000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information PHKG1(Phosphorylase b kinase gamma catalytic chain, skeletal muscle isoform) is also named as phosphorylase kinase subunit gamma-1, PHKG and belongs to the CAMK Ser/Thr protein kinase family. Phosphorylase kinase is a crucial glycogenolytic regulatory enzyme. The holoenzymes are composed of 16 subunit proteins containing equimolar ratios of 4 subunit types known as alpha, beta, gamma, and delta. This protein is the skeletal muscle isoform of phosphorylase kinase and kinetic differences exist between the cardiac and adult skeletal muscle enzymes, and the liver and skeletal muscle enzymes are known to be separate isozymes. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: pig Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag10032 Product name: Recombinant human PHKG1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-387 aa of BC051327 Sequence: MTRDEALPDSHSAQDFYENYEPKEILGRGVSSVVRRCIHKPTSQEYAVKVIDVTGGGSFSPEEVRELREATLKEVDILRKVSGHPNIIQLKDTYETNTFFFLVFDLMKRGELFDYLTEKVTLSEKETRKIMRALLEVICTLHKLNIVHRDLKPENILLDDNMNIKLTDFGFSCQLEPGERLREVCGTPSYLAPEIIECSMNEDHPGYGKEVDMWSTGVIMYTLLAGSPPFWHRKQMLMLRMIMSGNYQFGSPEWDDYSDTVKDLVSRFLVVQPQNRYTAEEALAHPFFQQYLVEEVRHFSPRGKFKVIALTVLASVRIYYQYRRVKPVTREIVIRDPYALRPLRRLIDAYAFRIYGHWVKKGQQQNRAALFENTPKAVLLSLAEEDY Predict reactive species Full Name: phosphorylase kinase, gamma 1 (muscle) Calculated Molecular Weight: 387 aa, 45 kDa Observed Molecular Weight: 40 kDa GenBank Accession Number: BC051327 Gene Symbol: PHKG1 Gene ID (NCBI): 5260 RRID: AB_1851532 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q16816 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.