Proteintech, 16770-1-AP, SPAG11A/B Polyclonal antibody

Size: 20ul / 150ul The SPAG11A/B (16770-1-AP) by Proteintech is a Polyclonal antibody targeting SPAG11A/B in WB, IHC, ELISA applications with reactivity to human samples 16770-1-AP targets SPAG11A/B in WB, IHC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: human testis tissue, human brain tissue Positive IHC detected in: human testis tissue, human kidney tissue, human placenta tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunohistochemistry (IHC): IHC : 1:20-1:200 Background Information Sperm binding proteins and their C-terminal peptides of the Sperm Associated Antigen 11 (SPAG11) family are found to play an important role in epididymal innate immunity in addition to their role in sperm maturation(PMID:16643671). SPAG11 peptides resulting due to cleavage at the furin-like proprotein convertase motif are reported in the epididymal fluid, ejaculate and on sperm and their antimicrobial activity demonstrated.SPAG11A belongs to this family and is also named as EP2,HE2. It has 4 isoforms produced by alternative splicing. This antibody recognizes SPAG11A and SPAG11B, but not SPAG11, because SPAG11 is a different transcript. Specification Tested Reactivity: human Cited Reactivity: mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag9846 Product name: Recombinant human SPAG11A protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-123 aa of BC058833 Sequence: MRQRLLPSVTSLLLVALLFPGSSQARHVNHSATEALGELRERAPGQGTNGFQLLRHAVKWDLLPPRTPPYQVHISHQEARGPSFKICVGFLGPRWARGCSTGNEKYHLPYAARDLQTFFLPFW Predict reactive species Full Name: sperm associated antigen 11A Calculated Molecular Weight: 123 aa, 14 kDa Observed Molecular Weight: 20 kDa GenBank Accession Number: BC058833 Gene Symbol: SPAG11A Gene ID (NCBI): 653423 RRID: AB_2286582 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q6PDA7 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.