Proteintech, 16782-1-AP, THEM2 Polyclonal antibody

Size: 20ul / 150ul The THEM2 (16782-1-AP) by Proteintech is a Polyclonal antibody targeting THEM2 in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human, mouse, rat samples 16782-1-AP targets THEM2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HeLa cells, HepG2 cells, mouse kidney tissue, mouse liver tissue Positive IP detected in: HeLa cells Positive IHC detected in: human lung cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:5000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information Them2 (also named Acot13) is a homotetrameric fatty acyl-CoA thioesterase. Them2 is enriched in liver and oxidative tissues, including BAT, and is primarily associated with mitochondria. Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag10212 Product name: Recombinant human THEM2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-140 aa of BC000894 Sequence: MTSMTQSLREVIKAMTKARNFERVLGKITLVSAAPGKVICEMKVEEEHTNAIGTLHGGLTATLVDNISTMALLCTERGAPGVSVDMNITYMSPAKLGEDIVITAHVLKQGKTLAFTSVDLTNKATGKLIAQGRHTKHLGN Predict reactive species Full Name: thioesterase superfamily member 2 Calculated Molecular Weight: 140 aa, 15 kDa Observed Molecular Weight: 16 kDa GenBank Accession Number: BC000894 Gene Symbol: THEM2 Gene ID (NCBI): 55856 RRID: AB_2287241 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9NPJ3 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.