Proteintech, 16791-1-AP, METTL5 Polyclonal antibody

Size: 20ul / 150ul The METTL5 (16791-1-AP) by Proteintech is a Polyclonal antibody targeting METTL5 in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human samples 16791-1-AP targets METTL5 in WB, IHC, IF/ICC, IP, RIP, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HEK-293 cells, HeLa cells, K-562 cells, PC-3 cells Positive IP detected in: PC-3 cells Positive IHC detected in: rat kidney tissue, mouse kidney tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:2000-1:12000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:400-1:1000 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information METTL5 is a member of the METTL family of S-adenosyl-methionine-dependent methyltransferases that adds m6A to a specific site, nucleotide A-1832 of 18S rRNA, hereafter referred to as m6A1832. METTL5 is localized in the nucleolus and cytoplasm. Specification Tested Reactivity: human Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag10232 Product name: Recombinant human METTL5 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-205 aa of BC000921 Sequence: MKKVRLKELESRLQQVDGFEKPKLLLEQYPTRPHIAACMLYTIHNTYDDIENKVVADLGCGCGVLSIGTAMLGAGLCVGFDIDEDALEIFNRNAEEFELTNIDMVQCDVCLLSNRMSKSFDTVIMNPPFGTKNNKGTDMAFLKTALEMARTAVYSLHKSSTREHVQKKAAEWKIKIDIIAELRYDLPASYKFHKKKSVDIEVDLI Predict reactive species Full Name: methyltransferase like 5 Calculated Molecular Weight: 209 aa, 24 kDa Observed Molecular Weight: 24-27 kDa GenBank Accession Number: BC000921 Gene Symbol: METTL5 Gene ID (NCBI): 29081 RRID: AB_2142051 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9NRN9 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.