Proteintech, 16796-1-AP, MPP8 Polyclonal antibody

Size: 20ul / 150ul The MPP8 (16796-1-AP) by Proteintech is a Polyclonal antibody targeting MPP8 in WB, IF/ICC, IP, ELISA applications with reactivity to human, mouse, rat samples 16796-1-AP targets MPP8 in WB, IHC, IF/ICC, IP, CoIP, chIP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HEK-293 cells, HeLa cells, HepG2 cells, MCF-7 cells, U-251 cells, NIH/3T3 cells, mouse brain tissue, rat brain tissue Positive IP detected in: HEK-293 cells Positive IF/ICC detected in: U2OS cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information The M-phase phosphoprotein 8 (MPP8) was firstly identified to coimmunoprecipitate with the RanBPM-comprised large protein complex [PMID:19956676]. It also binds to methylated H3K9 and recruited the H3K9 methyltransferases GLP and ESET, as well as DNA methyltransferase 3A (DNMT3A) to the promoter of the E-cadherin gene, a key regulator of tumor cell growth and epithelial-to-mesenchymal transition (EMT) [PMID:18726070]. This binding event of MPP8-H3K9me modulates the expression of E-cadherin, a key regulator of cell-cell adhesion with implications in embryonic development, tissue morphogenesis and homeostasis , together with H3K9 methyltransferases GLP and ESET and DNA methyltransferase 3a (Dnmt3a) [PMID: 18726070]. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag10268 Product name: Recombinant human MPHOSPH8 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-301 aa of BC003542 Sequence: MEQVAEGARVTAVPVSAADSTEELAEVEEGVGVVGEDNDAAARGAEAFGDSEEDGEDVFEVEKILDMKTEGGKVLYKVRWKGYTSDDDTWEPEIHLEDCKEVLLEFRKKIAENKAKAVRKDIQRLSLNNDIFEANSDSDQQSETKEDTSPKKKKKKLRQREEKSPDDLKKKKAKAGKLKDKSKPDLESSLESLVFDLRTKKRISEAKEELKESKKPKKDEVKETKELKKVKKGEIRDLKTKTREDPKENRKTKKEKFVESQVESESSVLNDSPFPEDDSEGLHSDSREEKQNTKSARERAG Predict reactive species Full Name: M-phase phosphoprotein 8 Calculated Molecular Weight: 860 aa, 97 kDa Observed Molecular Weight: 97-105 kDa GenBank Accession Number: BC003542 Gene Symbol: MPP8 Gene ID (NCBI): 54737 RRID: AB_2266644 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q99549 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.