Proteintech, 16813-1-AP, HSP20 Polyclonal antibody

Size: 20ul / 150ul The HSP20 (16813-1-AP) by Proteintech is a Polyclonal antibody targeting HSP20 in WB, IHC, IF-P, ELISA applications with reactivity to human, rat, mouse samples 16813-1-AP targets HSP20 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, rat, mouse samples. Tested Applications Positive WB detected in: rat heart tissue, mouse heart tissue Positive IHC detected in: mouse heart tissue, mouse brain tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF-P detected in: mouse heart tissue Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Immunohistochemistry (IHC): IHC : 1:50-1:200 Immunofluorescence (IF)-P: IF-P : 1:50-1:500 Background Information Hsp20 is a member of the small heat shock superfamily of proteins that function as chaperones to prevent protein misfolding through adenosine triphosphate−independent processes. It is widely expressed but is most abundant in cardiac, skeletal, and smooth muscles. Phosphorylation of HSP20 at Ser16 was elevated in human failing hearts as well as in murine hearts after I/R injury Indicating its cardioprotective function. Specification Tested Reactivity: human, rat, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag10352 Product name: Recombinant human HSPB6 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-160 aa of BC068046 Sequence: MEIPVPVQPSWLRRASAPLLGLSAPGRLFDQRFGEGLLEAELAALCPTTLAPYYLRAPSVALPVAQVPTDPGHFSVLLDVKHFSPEEIAVKVVGEHVEVHARHEERPDEHGFVAREFHRRYRLPPGVDPAAVTSALSPEGVLSIQAAPASAQAPPPAAAK Predict reactive species Full Name: heat shock protein, alpha-crystallin-related, B6 Calculated Molecular Weight: 160 aa, 17 kDa Observed Molecular Weight: 18-20 kDa GenBank Accession Number: BC068046 Gene Symbol: HSP20 Gene ID (NCBI): 126393 RRID: AB_2878321 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: O14558 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.