Proteintech, 16826-1-AP, PIK3IP1 Polyclonal antibody

Size: 20ul / 150ul The PIK3IP1 (16826-1-AP) by Proteintech is a Polyclonal antibody targeting PIK3IP1 in WB, IP, ELISA applications with reactivity to human, mouse, rat samples 16826-1-AP targets PIK3IP1 in WB, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HeLa cells, HepG2 cells, mouse liver tissue Positive IP detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Background Information PIK3IP1(Phosphoinositide-3-kinase-interacting protein 1) is also named as HGFL. The class IA phosphoinositol-3-kinases (PI3Ks) regulate important cellular processes such as proliferation, growth, survival, motility and metabolism. PIK3IP1 is a transmembrane protein that possesses a region in its intracellular domain that shares homology with the p85 regulatory subunit of PI3K. It has 5 isoforms produced by alternative splicing with the MW of 11, 18, 19, 25,28 kDa. PIK3IP1 undergoes N- and O-linked amino acid glycosylation. The major glycosylated form of PIK3IP1 migrates at about 43 kDa in western blot analysis, while the unglycosylated form migrates at 37 kDa. It is also detected a 65 kDa variant in HepG2 cell lysate that PIK3IP1 is indeed membrane bound and that these variants arise from alternative splicing and/or post-translational processing events such as enzymatic proteolysis and/or glycosylation. (PMID:18632611). Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, rat, chicken, bovine Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag10427 Product name: Recombinant human PIK3IP1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 24-263 aa of BC011049 Sequence: GCFWDNGHLYREDQTSPAPGLRCLNWLDAQSGLASAPVSGAGNHSYCRNPDEDPRGPWCYVSGEAGVPEKRPCEDLRCPETTSQALPAFTTEIQEASEGPGADEVQVFAPANALPARSEAAAVQPVIGISQRVRMNSKEKKDLGTLGYVLGITMMVIIIAIGAGIILGYSYKRGKDLKEQHDQKVCEREMQRITLPLSAFTNPTCEIVDEKTVVVHTSQTPVDPQEGSTPLMGQAGTPGA Predict reactive species Full Name: phosphoinositide-3-kinase interacting protein 1 Calculated Molecular Weight: 263 aa, 28 kDa Observed Molecular Weight: 37 kDa GenBank Accession Number: BC011049 Gene Symbol: PIK3IP1 Gene ID (NCBI): 113791 RRID: AB_2163333 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q96FE7 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.