Proteintech, 16840-1-AP, USP13 Polyclonal antibody

Size: 20ul / 150ul The USP13 (16840-1-AP) by Proteintech is a Polyclonal antibody targeting USP13 in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human, mouse, rat samples 16840-1-AP targets USP13 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HeLa cells, mouse testis tissue, A375 cells, HEK-293 cells, rat testis tissue, PC-3 cells Positive IP detected in: HeLa cells Positive IHC detected in: human skin cancer tissue, human brain tissue, human heart tissue, human liver tissue, human ovary tissue, human placenta tissue, human testis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:1000-1:6000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information USP13(Ubiquitin carboxyl-terminal hydrolase 13) is also named as ISOT3 and belongs to the peptidase C19 family. It is involved in ubiquitin-dependent protein catabolism. USP13 is responsible for MITF deubiquitination, utilizing a short hairpin RNA library against known deubiquitinating enzymes. This protein has 2 isoforms produced by alternative splicing. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag10527 Product name: Recombinant human USP13 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 515-863 aa of BC016146 Sequence: IAYELTRREAEANRRPLPELVRAKIPFSACLQAFSEPENVDDFWSSALQAKSAGVKTSRFASFPEYLVVQIKKFTFGLDWVPKKFDVSIDMPDLLDINHLRARGLQPGEEELPDISPPIVIPDDSKDRLMNQLIDPSDIDESSVMQLAEMGFPLEACRKAVYFTGNMGAEVAFNWIIVHMEEPDFAEPLTMPGYGGAASAGASVFGASGLDNQPPEEIVAIITSMGFQRNQAIQALRATNNNLERALDWIFSHPEFEEDSDFVIEMENNANANIISEAKPEGPRVKDGSGTYELFAFISHMGTSTMSGHYICHIKKEGRWVIYNDHKVCASERPPKDLGYMYFYRRIPS Predict reactive species Full Name: ubiquitin specific peptidase 13 (isopeptidase T-3) Calculated Molecular Weight: 863 aa, 97 kDa Observed Molecular Weight: 97-100 kDa GenBank Accession Number: BC016146 Gene Symbol: USP13 Gene ID (NCBI): 8975 RRID: AB_2214569 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q92995 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.