Proteintech, 16895-1-AP, RETSAT Polyclonal antibody

Size: 20ul / 150ul The RETSAT (16895-1-AP) by Proteintech is a Polyclonal antibody targeting RETSAT in WB, ELISA applications with reactivity to human samples 16895-1-AP targets RETSAT in WB, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HeLa cells, HepG2 cells, L02 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:6000 Background Information RETSAT (Retinol Saturase) is an enzyme-encoding gene that plays a critical role in cellular metabolism, primarily involved in the saturation of retinol (vitamin A) and other molecules. It catalyzes the conversion of all-trans-retinol to all-trans-13,14-dihydroretinol, a process that links vitamin A metabolism to lipid and glucose homeostasis. It is highly expressed in the liver, adipose tissue, and kidney and is regulated by the PPARγ master regulator of adipogenesis (PMID: 28855500; 38128827). RETSAT also has a role in cancer cell survival, particularly in hypoxic tumor environments, by enhancing resistance to replication stress (PMID: 36109793). Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag10420 Product name: Recombinant human RETSAT protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-313 aa of BC011418 Sequence: MYSGEKAYIQGLKEKFPQEEAIIDKYIKLVKVVSSGAPHAILLKFLPLPVVQLLDRCGLLTRFSPFLQASTQSLAEVLQQLGASSELQAVLSYIFPTYGVTPNHSAFSMHALLVNHYMKGGFYPRGGSSQIAFHTIPVIQRAGGAVLTKATVQSVLLDSAGKACGVSVKKGHELVNIYCPIVVSNAGLFNTYEHLLPGNARCLPGVKQQLGTVRPGLGMTSVFICLRGTKEDLHLPSTNYYVYYDTDMDQAMERYVSMPREEAAEHIPLLFFAFPSAKDPTWEDRFPGGECDCRIPTHQPVLSGCSPRCLLRG Predict reactive species Full Name: retinol saturase (all-trans-retinol 13,14-reductase) Calculated Molecular Weight: 610 aa, 69 kDa Observed Molecular Weight: 62-67 kDa GenBank Accession Number: BC011418 Gene Symbol: RETSAT Gene ID (NCBI): 54884 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q6NUM9 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.