Proteintech, 16898-1-AP, HMGCL Polyclonal antibody

Size: 20ul / 150ul The HMGCL (16898-1-AP) by Proteintech is a Polyclonal antibody targeting HMGCL in WB, IHC, IP, ELISA applications with reactivity to human, mouse, rat samples 16898-1-AP targets HMGCL in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: mouse liver tissue, mouse brain tissue, human heart tissue, rat liver tissue Positive IP detected in: mouse spleen tissue Positive IHC detected in: human liver tissue, human ovary tissue, human spleen tissue, human testis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:1000-1:6000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:20-1:200 Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag10426 Product name: Recombinant human HMGCL protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-325 aa of BC010570 Sequence: MAAMRKALPRRLVGLASLRAVSTSSMGTLPKRVKIVEVGPRDGLQNEKNIVSTPVKIKLIDMLSEAGLSVIETTSFVSPKWVPQMGDHTEVLKGIQKFPGINYPVLTPNLKGFEAAVAAGAKEVVIFGAASELFTKKNINCSIEESFQRFDAILKAAQSANISVRGYVSCALGCPYEGKISPAKVAEVTKKFYSMGCYEISLGDTIGVGTPGIMKDMLSAVMQEVPLAALAVHCHDTYGQALANTLMALQMGVSVVDSSVAGLGGCPYAQGASGNLATEDLVYMLEGLGIHTGVNLQKLLEAGNFICQALNRKTSSKVAQATCKL Predict reactive species Full Name: 3-hydroxymethyl-3-methylglutaryl-Coenzyme A lyase Calculated Molecular Weight: 325 aa, 34 kDa Observed Molecular Weight: 34 kDa GenBank Accession Number: BC010570 Gene Symbol: HMGCL Gene ID (NCBI): 3155 RRID: AB_2295304 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P35914 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.