Proteintech, 16903-1-AP, UBR7 Polyclonal antibody

Size: 20ul / 150ul The UBR7 (16903-1-AP) by Proteintech is a Polyclonal antibody targeting UBR7 in WB, IF/ICC, ELISA applications with reactivity to human samples 16903-1-AP targets UBR7 in WB, IF/ICC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: DU 145 cells, HeLa cells, LNCaP cells, PC-3 cells Positive IF/ICC detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600 Background Information UBR7 (C14orf130), is a 425-amino-acid putative E3 ubiquitin-protein ligase which is a component of the N-end rule pathway. The N-end rule pathway is a ubiquitin-dependent system where E3 ligases called N-recognins, including UBR1, UBR2 and UBR7 (N-recognin-7), leading to target protein ubiquitination and subsequent degradation (PMID: 19008229). UBR7 contains a PHD-type zinc finger and a UBR-type zinc finger domain (PMID: 14702039), and probably undergoes acetylation (PMID: 19413330). Till date its precise function is not well known. Proteintech's UBR7 antibody 16903-1-AP is able to detect UBR7 with a theoretical MW of 48 kDa. Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag10445 Product name: Recombinant human UBR7 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-349 aa of BC015046 Sequence: ACSYECHGSHKLFELYTKRNFRCDCGNSKFKNLECKLLPDKAKVNSGNKYNDNFFGLYCICKRPYPDPEDEIPDEMIQCVVCEDWFHGRHLGAIPPESGDFQEMVCQACMKRCSFLWAYAAQLAVTKISTEDDGLVRNIDGIGDQEVIKPENGEHQDSTLKEDVPEQGKDDVREVKVEQNSEPCAGSSSESDLQTVFKNESLNAESKSGCKLQELKAKQLIKKDTATYWPLNWRSKLCTCQDCMKMYGDLDVLFLTDEYDTVLAYENKGKIAQATDRSDPLMDTLSSMNRVQQVELICEYNDLKTELKDYLKRFADEGTVVKREDIQQFFEEFQSKKRRRVDGMQYYCS Predict reactive species Full Name: ubiquitin protein ligase E3 component n-recognin 7 (putative) Calculated Molecular Weight: 48 kDa Observed Molecular Weight: 48-60 kDa GenBank Accession Number: BC015046 Gene Symbol: UBR7 Gene ID (NCBI): 55148 RRID: AB_2256707 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q8N806 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.