Proteintech, 17050-1-AP, PCDHB13 Polyclonal antibody

Size: 20ul / 150ul The PCDHB13 (17050-1-AP) by Proteintech is a Polyclonal antibody targeting PCDHB13 in IHC, IP, ELISA applications with reactivity to human, mouse samples 17050-1-AP targets PCDHB13 in IHC, IP, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive IP detected in: A375 cells Positive IHC detected in: mouse brain tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information Protocadherin beta-13 (PCDHB13) is a potential calcium-dependent cell-adhesion protein. PCDHB13 may be involved in the establishment and maintenance of specific neuronal connections in the brain. PCDHB13 is highly expressed on the melanoma cell surface (PMID: 27068704). The calculated molecular weight of PCDHB13 is 88 kDa. Specification Tested Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag10713 Product name: Recombinant human PCDHB13 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 22-353 aa of BC033068 Sequence: GLSLAGAAEPRSYSVVEETEGSSFVTNLAKDLGLEQREFSRRGVRVVSRGNKLHLQLNQETADLLLNEKLDREDLCGHTEPCVLRFQVLLESPFEFFQAELQVIDINDHSPVFLDKQMLVKVSESSPPGTTFPLKNAEDLDVGQNNIENYIISPNSYFRVLTRKRSDGRKYPELVLDKALDREEEAELRLTLTALDGGSPPRSGTAQVYIEVLDVNDNAPEFEQPFYRVQISEDSPVGFLVVKVSATDVDTGVNGEISYSLFQASEEIGKTFKINPLTGEIELKKQLDFEKLQSYEVNIEARDAGTFSGKCTVLIQVIDVNDHAPEVTMSAF Predict reactive species Full Name: protocadherin beta 13 Calculated Molecular Weight: 798 aa, 88 kDa Observed Molecular Weight: 88 kDa GenBank Accession Number: BC033068 Gene Symbol: PCDHB13 Gene ID (NCBI): 56123 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9Y5F0 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.