Proteintech, 17073-1-AP, LZTFL1 Polyclonal antibody

Size: 20ul / 150ul The LZTFL1 (17073-1-AP) by Proteintech is a Polyclonal antibody targeting LZTFL1 in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse, rat samples 17073-1-AP targets LZTFL1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HEK-293 cells, HeLa cells, HepG2 cells, U-251 cells, mouse testis tissue, rat testis tissue Positive IHC detected in: human colon tissue, human heart tissue, human lung tissue, human small intestine tissue, human testis tissue, mouse heart tissue, mouse lung tissue, mouse small intestine tissue, mouse testis tissue, rat heart tissue, rat small intestine tissue, rat testis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:2000-1:12000 Immunohistochemistry (IHC): IHC : 1:250-1:1000 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information LZTFL1 belongs to the LZTFL1 family. It is an important regulator of BBSome ciliary trafficking and hedgehog signaling. LZTFL1 regulates Ciliary Trafficking of the BBSome and Smoothened.(PMID:22072986) Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag10919 Product name: Recombinant human LZTFL1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-299 aa of BC025988 Sequence: MAELGLNEHHQNEVINYMRFARSKRGLRLKTVDSCFQDLKESRLVEDTFTIDEVSEVLNGLQAVVHSEVESELINTAYTNVLLLRQLFAQAEKWYLKLQTDISELENQELLEQVAEFEKAEITSSNKKPILDVTKPKLAPLNEGGTAELLNEEILRLQEENEKLKSRLKTIEIQATNALDEKSKLEKALQDLQLDQGNQKDFIKAQDLSNLENTVAALKSEFQKTLNDKTENQKSLEENLATAKHDLLRVREQLHMAEKELEKKFQQTAAYRNMKEILTKKNDQIKDLRKRLAQYEPED Predict reactive species Full Name: leucine zipper transcription factor-like 1 Calculated Molecular Weight: 299 aa, 35 kDa Observed Molecular Weight: 35 kDa GenBank Accession Number: BC025988 Gene Symbol: LZTFL1 Gene ID (NCBI): 54585 RRID: AB_10950400 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9NQ48 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.