Proteintech, 17082-1-AP, RPL24 Polyclonal antibody

Size: 20ul / 150ul The RPL24 (17082-1-AP) by Proteintech is a Polyclonal antibody targeting RPL24 in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human samples 17082-1-AP targets RPL24 in WB, IHC, IF/ICC, IP, RIP, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: A549 cells, HEK-293 cells, Jurkat cells Positive IP detected in: HEK-293 cells Positive IHC detected in: human placenta tissue, human kidney tissue, human liver tissue, human spleen tissue, human ovary tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: Hela cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100 Background Information The mammalian ribosome comprises 79 ribosomal proteins and four rRNAs, which combine in equimolar ratios to form the small (40S) and large (60S) subunits. Ribosome proteins are a direct and critical target of the PI3K pathway in promoting growth.[PMID:15289434]. RPL24 is one component of the large (60S) subunits that promote the translation of uORF-containing mRNAsgene The mutation in Rpl24 result in impairment of mRNA splicing and L24 production, which in turn affects ribosome biogenesis, protein synthesis and the cell cycle. PMID:20799971]. Also RPL24 (ribosomal protein L24) is a key factor for translation reinitiation of downstream ORFs on the polycistronic cauliflower mosaic virus 35S RNA transcription unit, and may have a role in gynoecuim development.[PMID:15270688] Specification Tested Reactivity: human Cited Reactivity: human, mouse, xenopus Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag7085 Product name: Recombinant human RPL24 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-157 aa of BC000690 Sequence: MKVELCSFSGYKIYPGHGRRYARTDGKVFQFLNAKCESAFLSKRNPRQINWTVLYRRKHKKGQSEEIQKKRTRRAVKFQRAITGASLADIMAKRNQKPEVRKAQREQAIRAAKEAKKAKQASKKTAMAAAKAPTKAAPKQKIVKPVKVSAPRVGGKR Predict reactive species Full Name: ribosomal protein L24 Calculated Molecular Weight: 18 kDa Observed Molecular Weight: 21-23 kDa GenBank Accession Number: BC000690 Gene Symbol: RPL24 Gene ID (NCBI): 6152 RRID: AB_2181728 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P83731 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.