Proteintech, 17221-1-AP, HLA-DRA Polyclonal antibody

Size: 20ul / 150ul The HLA-DRA (17221-1-AP) by Proteintech is a Polyclonal antibody targeting HLA-DRA in WB, IHC, IF/ICC, IF-P, IP, ELISA applications with reactivity to human, rat samples 17221-1-AP targets HLA-DRA in WB, IHC, IF/ICC, IF-P, IP, ELISA applications and shows reactivity with human, rat samples. Tested Applications Positive WB detected in: Daudi cells, rat spleen tissue, Raji cells, Ramos cells Positive IP detected in: Raji cells Positive IHC detected in: human appendicitis tissue, human tonsillitis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF-P detected in: human tonsillitis tissue Positive IF/ICC detected in: Raji cells Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:500-1:2000 Immunofluorescence (IF)-P: IF-P : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Specification Tested Reactivity: human, rat Cited Reactivity: human, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag11044 Product name: Recombinant human HLA-DRA protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 24-218 aa of BC032350 Sequence: WAIKEEHVIIQAEFYLNPDQSGEFMFDFDGDEIFHVDMAKKETVWRLEEFGRFASFEAQGALANIAVDKANLEIMTKRSNYTPITNVPPEVTVLTNSPVELREPNVLICFIDKFTPPVVNVTWLRNGKPVTTGVSETVFLPREDHLFRKFHYLPFLPSTEDVYDCRVEHWGLDEPLLKHWEFDAPSPLPETTENV Predict reactive species Full Name: major histocompatibility complex, class II, DR alpha Calculated Molecular Weight: 254 aa, 29 kDa Observed Molecular Weight: 32-34 kDa GenBank Accession Number: BC032350 Gene Symbol: HLA-DRA Gene ID (NCBI): 3122 ENSEMBL Gene ID: ENSG00000204287 RRID: AB_10639510 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P01903 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.