Proteintech, 17240-1-AP, NSUN6 Polyclonal antibody

Size: 20ul / 150ul The NSUN6 (17240-1-AP) by Proteintech is a Polyclonal antibody targeting NSUN6 in WB, IHC, ELISA applications with reactivity to human, mouse, rat samples 17240-1-AP targets NSUN6 in WB, IHC, IF, RIP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: Neuro-2a cells, HEK-293 cells, HepG2 cells, mouse brain Positive IHC detected in: human colon cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunohistochemistry (IHC): IHC : 1:500-1:2000 Background Information NSUN6 is an RNA methyltransferase that catalyzes the methylation at position cytosine C72 in some tRNAs. NSUN6 has been reported to localize to the cytoplasm and largely colocalize with the Golgi apparatus and pericentriolar matrix, indicating that NSUN6 modifies tRNAs in a late step in their biogenesis. NSUN6 may also play a major role in bone metastasis and could be a valuable therapeutic target for bone metastasis. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag11084 Product name: Recombinant human NSUN6 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 118-469 aa of BC035778 Sequence: AQCGNAVLRGAHVYAPGIVSASQFMKAGDVISVYSDIKGKCKKGAKEFDGTKVFLGNGISELSRKEIFSGLPELKGMGIRMTEPVYLSPSFDSVLPRYLFLQNLPSALVSHVLNPQPGEKILDLCAAPGGKTTHIAALMHDQGEVIALDKIFNKVEKIKQNALLLGLNSIRAFCFDGTKAVKLDMVEDTEGEPPFLPESFDRILLDAPCSGMGQRPNMACTWSVKEVASYQPLQRKLFTAAVQLLKPEGVLVYSTCTITLAENEEQVAWALTKFPCLQLQPQEPQIGGEGMRGAGLSCEQLKQLQRFDPSAVPLPDTDMDSLREARREDMLRLANKDSIGFFIAKFVKCKST Predict reactive species Full Name: NOL1/NOP2/Sun domain family, member 6 Calculated Molecular Weight: 469 aa, 52 kDa Observed Molecular Weight: 52-55 kDa GenBank Accession Number: BC035778 Gene Symbol: NSUN6 Gene ID (NCBI): 221078 RRID: AB_2878367 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q8TEA1 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.