Proteintech, 17382-1-AP, DNTT,TDT Polyclonal antibody

Size: 20ul / 150ul The DNTT,TDT (17382-1-AP) by Proteintech is a Polyclonal antibody targeting DNTT,TDT in IHC, FC (Intra), IP, ELISA applications with reactivity to human samples 17382-1-AP targets DNTT,TDT in IHC, FC (Intra), IP, ELISA applications and shows reactivity with human samples. Tested Applications Positive IP detected in: Raji cells Positive IHC detected in: human lymphoma tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive FC (Intra) detected in: Jurkat cells Recommended dilution Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:20-1:200 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag11235 Product name: Recombinant human DNTT,TDT protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 162-509 aa of BC012920 Sequence: NNCNQIFTDAFDILAENCEFRENEDSCVTFMRAASVLKSLPFTIISMKDTEGIPCLGSKVKGIIEEIIEDGESSEVKAVLNDERYQSFKLFTSVFGVGLKTSEKWFRMGFRTLSKVRSDKSLKFTRMQKAGFLYYEDLVSCVTRAEAEAVSVLVKEAVWAFLPDAFVTMTGGFRRGKKMGHDVDFLITSPGSTEDEEQLLQKVMNLWEKKGLLLYYDLVESTFEKLRLPSRKVDALDHFQKCFLIFKLPRQRVDSDQSSWQEGKTWKAIRVDLVLCPYERRAFALLGWTGSRQFERDLRRYATHERKMILDNHALYDKTKRIFLKAESEEEIFAHLGLDYIEPWERNA Predict reactive species Full Name: deoxynucleotidyltransferase, terminal Calculated Molecular Weight: 509 aa, 58 kDa GenBank Accession Number: BC012920 Gene Symbol: DNTT Gene ID (NCBI): 1791 RRID: AB_2878399 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P04053 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.