Proteintech, 17435-1-AP, Transferrin/TF Polyclonal antibody

Size: 150ul The Transferrin/TF (17435-1-AP) by Proteintech is a Polyclonal antibody targeting Transferrin/TF in WB, IHC, IF/ICC, IF-P, ELISA applications with reactivity to human, mouse, rat samples 17435-1-AP targets Transferrin/TF in WB, IHC, IF/ICC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: human urine sample, HepG2 cells, mouse kidney tissue, mouse spleen tissue, rat kidney tissue Positive IHC detected in: human hepatocirrhosis tissue, human placenta tissue, human testis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF-P detected in: human placenta tissue Positive IF/ICC detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:5000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)-P: IF-P : 1:200-1:800 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information What is the tissue specificity of TF?Transferrin is synthesized mainly in the liver.What is the calculated molecular weight of TF?77 kDaHow many iron atoms can bind to TF?Each of the two lobes of TF has one high-affinity Fe(III) binding site. Since binding of ironand bicarbonatereleasesfree H+ ions, affinity decreases in acidic conditions, in which TFalso remains bound to its receptor.What is the primary role of TF?TF serves as a serum metal-binding protein, playing a key role in iron metabolism anddelivery for erythropoiesis.What is the role of TF in human diseases?TF deficiency results in an exhibition of congenital atransferrinemia (hypotransferrinemia)in which there is adecrease in hepcidin expression, causing increased iron levels.Is TF essential for iron delivery?Congenital atransferrinemia results in an iron overload in some tissues, such as the liver,suggesting that while  TF is not essential for iron delivery to all organs, it is needed for iron level regulation.In what circumstances do TF levels decrease?Inflammation and iron overload both can cause a decline in TF levels.How does TF affect hormonal balance?TF positively regulates expression of hepcidin, a peptide hormone synthesized by the liver.How does TF deficiency cause increased iron levels?TF regulates hepcidin, which in turn is a negative regulator of intestinal iron absorption,resulting in TFdeficiency causing both anemia, and, paradoxically, increased iron levelsin some tissues.PMIDs: 23046645, 9635730, 22294463, 24589273 Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, rat, pig, monkey, bovine, duck Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag11504 Product name: Recombinant human TF protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 347-698 aa of BC059367 Sequence: EGTCQEAPTDECKPVKWCALSHHERLKCDEWSVNSVGKIECVSAETTEDCIAKIMNGEADAMSLDGGFVYIAGKCGLVPVLAENYNKSDNCEDTPEAGYFAVAVVKKSASDLTWDNLKGKKSCHTAVGRTAGWNIPMGLLYNKINHCRFDEFFSEGCAPGSKKDSSLCKLCMGSGLNLCEPNNKEGYYGYTGAFRCLVEKGDVAFVKHQTVPQNTGGKNPDPWAKNLNEKDYELLCLDGTRKPVEEYANCHLARAPNHAVVTRKDKEACVHKILRQQQHLFGSNVTDCSGNFCLFRSETKDLLFRDDTVCLAKLHDRNTYEKYLGEEYVKAVGNLRKCSTSSLLEACTFRRP Predict reactive species Full Name: transferrin Calculated Molecular Weight: 698 aa, 77 kDa Observed Molecular Weight: 77 kDa GenBank Accession Number: BC059367 Gene Symbol: TF Gene ID (NCBI): 7018 RRID: AB_2035023 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P02787 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.