Proteintech, 17470-1-AP, CLUAP1 Polyclonal antibody

Size: 20ul / 150ul The CLUAP1 (17470-1-AP) by Proteintech is a Polyclonal antibody targeting CLUAP1 in WB, IF/ICC, IP, ELISA applications with reactivity to human, mouse, rat, canine samples 17470-1-AP targets CLUAP1 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat, canine samples. Tested Applications Positive WB detected in: mouse testis tissue, COLO 320 cells, HepG2 cells, Y79 cells Positive IP detected in: HepG2 cells Positive IF/ICC detected in: MDCK cells Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information CLUAP1 (Clusterin-associated protein 1) was originally identified as a protein that interacts with CLU/clusterin, and whose gene is frequently upregulated in colon cancer (PMID: 15480429). Overexpression of CLUAP1 was also observed in osteosarcoma (PMID: 17203229). CLUAP1 is most abundantly expressed in testis among normal tissues (PMID: 15480429; 17203229). Studies reveal that CLUAP1 has a role in ciliogenenesis and cilia-dependent development (PMID: 23351563; 23742838; 24970261). Specification Tested Reactivity: human, mouse, rat, canine Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag11419 Product name: Recombinant human CLUAP1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-413 aa of BC017070 Sequence: MSFRDLRNFTEMMRALGYPRHISMENFRTPNFGLVSEVLLWLVKRYEPQTDIPPDVDTEQDRVFFIKAIAQFMATKAHIKLNTKKLYQADGYAVKELLKITSVLYNAMKTKGMEGSEIVEEDVNKFKFDLGSKIADLKAARQLASEITSKGASLYDLLGMEVELREMRTEAIARPLEINETEKVMRIAIKEILTQVQKTKDLLNNVASDEANLEAKIEKRKLELERNRKRLETLQSVRPCFMDEYEKTEEELQKQYDTYLEKFQNLTYLEQQLEDHHRMEQERFEEAKNTLCLIQNKLKEEEKRLLKSGSNDDSDIDIQEDDESDSELEERRLPKPQTAMEMLMQGRPGKRIVGTMQGGDSDDNEDSEESEIDMEDDDDEDDDLEDESISLSPTKPNRRVWKSEPLDESDNDF Predict reactive species Full Name: clusterin associated protein 1 Calculated Molecular Weight: 413 aa, 48 kDa Observed Molecular Weight: 53 kDa GenBank Accession Number: BC017070 Gene Symbol: CLUAP1 Gene ID (NCBI): 23059 RRID: AB_2083477 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q96AJ1 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.