Proteintech, 17487-1-AP, NUDT12 Polyclonal antibody

Size: 20ul / 150ul The NUDT12 (17487-1-AP) by Proteintech is a Polyclonal antibody targeting NUDT12 in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse, rat samples 17487-1-AP targets NUDT12 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: mouse liver tissue, HeLa cells Positive IHC detected in: human lung tissue, mouse liver tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: MCF-7 cells, HeLa cells Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information NUDT12 is a member of the Nudix superfamily of pyrophosphohydrolases that has a broad range of substrates, including (d)NTPs, nucleotides, capped RNAs, dinucleotide coenzymes (NAD, FAD, dpCoA), and their derivatives. NUDT12 is active only as homodimers, with each monomer contributing to creation of the two functional catalytic pockets (PMID: 37301333, 31875550). The NUDT12 gene on chromosome 5q21 encodes the open reading frame of the putative 462-amino-acid, 52 kDa NUDT12 protein (PMID: 12790796). Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag11557 Product name: Recombinant human NUDT12 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-377 aa of BC041099 Sequence: MSSVKRSLKQEIVTQFHCSAAEGDIAKLTGILSHSPSLLNETSENGWTALMYAARNGHPEIVQFLLEKGCDRSIVNKSRQTALDIAVFWGYKHIANLLATAKGGKKPWFLTNEVEECENYFSKTLLDRKSEKRNNSDWLLAKESHPATVFILFSDLNPLVTLGGNKESFQQPEVRLCQLNYTDIKDYLAQPEKITLIFLGVELEIKDKLLNYAGEVPREEEDGLVAWFALGIDPIAAEEFKQRHENCYFLHPPMPALLQLKEKEAGVVAQARSVLAWHSRYKFCPTCGNATKIEEGGYKRLCLKEDCPSLNGVHNTSYPRVDPVVIMQVIHPDGTKCLLGRQKRFPPGMFTCLAGFIEPGETIEDAVRREVEEESGV Predict reactive species Full Name: nudix (nucleoside diphosphate linked moiety X)-type motif 12 Calculated Molecular Weight: 462 aa, 52 kDa Observed Molecular Weight: 45-50 kDa GenBank Accession Number: BC041099 Gene Symbol: NUDT12 Gene ID (NCBI): 83594 RRID: AB_2236164 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9BQG2 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.