Proteintech, 17521-1-AP, ACTA1-Specific Polyclonal antibody

Size: 20ul / 150ul The ACTA1-Specific (17521-1-AP) by Proteintech is a Polyclonal antibody targeting ACTA1-Specific in WB, IHC, FC (Intra), ELISA applications with reactivity to human, mouse, rat samples 17521-1-AP targets ACTA1-Specific in WB, IHC, IF, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: human skeletal muscle tissue, mouse skeletal muscle tissue, rat skeletal muscle tissue Positive IHC detected in: human skeletal muscle tissue, human hysteromyoma tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive FC (Intra) detected in: C2C12 cells Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension Background Information Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. The ACTA1 gene encodes skeletal muscle alpha-actin, the principal actin isoform in adult skeletal muscle, which interacts with a variety of proteins to produce the force for muscle contraction. There is only the N-termninal 8 amino acids difference among the actin family members. This antibody is specific to the ACTA1. It does not cross-react with other actin isoforms. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: actin, alpha 1, skeletal muscle Calculated Molecular Weight: 42 kDa Observed Molecular Weight: 39-42 kDa GenBank Accession Number: NM_001100 Gene Symbol: ACTA1 Gene ID (NCBI): 58 RRID: AB_2273608 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P68133 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.