Proteintech, 17530-1-AP, POLR3A Polyclonal antibody

Size: 20ul / 150ul The POLR3A (17530-1-AP) by Proteintech is a Polyclonal antibody targeting POLR3A in WB, IHC, ELISA applications with reactivity to human, mouse, rat samples 17530-1-AP targets POLR3A in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HeLa cells, MCF-7 cells Positive IHC detected in: mouse cerebellum tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Specification Tested Reactivity: human, mouse, rat Cited Reactivity: mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag11536 Product name: Recombinant human POLR3A protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1041-1390 aa of BC041089 Sequence: IGEPGTQMTLKTFHFAGVASMNITLGVPRIKEIINASKAISTPIITAQLDKDDDADYARLVKGRIEKTLLGEISEYIEEVFLPDDCFILVKLSLERIRLLRLEVNAETVRYSICTSKLRVKPGDVAVHGEAVVCVTPRENSKSSMYYVLQFLKEDLPKVVVQGIPEVSRAVIHIDEQSGKEKYKLLVEGDNLRAVMATHGVKGTRTTSNNTYEVEKTLGIEAARTTIINEIQYTMVNHGMSIDRRHVMLLSDLMTYKGEVLGITRFGLAKMKESVLMLASFEKTADHLFDAAYFGQKDSVCGVSECIIMGIPMNIGTGLFKLLHKADRDPNPPKRPLIFDTNEFHIPLVT Predict reactive species Full Name: polymerase (RNA) III (DNA directed) polypeptide A, 155kDa Calculated Molecular Weight: 1390 aa, 156 kDa Observed Molecular Weight: 140-150 kDa GenBank Accession Number: BC041089 Gene Symbol: POLR3A Gene ID (NCBI): 11128 RRID: AB_2167983 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: O14802 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.