Proteintech, 17534-1-AP, IFT52 Polyclonal antibody

Size: 20ul / 150ul The IFT52 (17534-1-AP) by Proteintech is a Polyclonal antibody targeting IFT52 in WB, IF/ICC, IP, ELISA applications with reactivity to human, mouse, rat, canine samples 17534-1-AP targets IFT52 in WB, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat, canine samples. Tested Applications Positive WB detected in: HEK-293 cells, human testis tissue, mouse testis tissue, MDCK cells, LNCaP cells Positive IP detected in: mouse testis tissue, HeLa cells Positive IF/ICC detected in: Hela cells Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100 Background Information IFT52, also named as C20orf9 and NGD5, is a component of IFT complex B which is composed of IFT88, IFT57, TRAF3IP1, IFT52, IFT27, HSPB11 and IFT20. IFT52 binds directly to the IFT81/74/27/25 complex. And IFT52 could mediate the interaction between the two subcomplexes.(PMID:21642430) The MW of IFT52 is about 45-54kd. Specification Tested Reactivity: human, mouse, rat, canine Cited Reactivity: human, mouse, rat, chicken Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag11586 Product name: Recombinant human IFT52 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-437 aa of BC039831 Sequence: MEKELRSTILFNAYKKEIFTTNNGYKSMQKKLRSNWKIQSLKDEITSEKLNGVKLWITAGPREKFTAAEFEILKKYLDTGGDVFVMLGEGGESRFDTNINFLLEEYGIMVNNDAVVRNVYHKYFHPKEALVSSGVLNREISRAAGKAVPGIIDEESSGNNAQALTFVYPFGATLSVMKPAVAVLSTGSVCFPLNRPILAFYHSKNQGGKLAVLGSCHMFSDQYLDKEENSKIMDVVFQWLTTGDIHLNQIDAEDPEISDYMMLPYTATLSKRNRECLQESDEIPRDFTTLFDLSIFQLDTTSFHSVIEAHEQLNVKHEPLQLIQPQFETPLPTLQPAVFPPSFRELPPPPLELFDLDETFSSEKARLAQITNKCTEEDLEFYVRKCGDILGVTSKLPKDQQDAKHILEHVFFQVVEFKKLNQEHDIDTSETAFQNNF Predict reactive species Full Name: intraflagellar transport 52 homolog (Chlamydomonas) Calculated Molecular Weight: 437 aa, 50 kDa Observed Molecular Weight: 45-50 kDa GenBank Accession Number: BC039831 Gene Symbol: IFT52 Gene ID (NCBI): 51098 RRID: AB_2280005 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9Y366 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.