Proteintech, 17640-1-AP, SPCS3 Polyclonal antibody

Size: 20ul / 150ul The SPCS3 (17640-1-AP) by Proteintech is a Polyclonal antibody targeting SPCS3 in WB, IHC, ELISA applications with reactivity to human, mouse, rat samples 17640-1-AP targets SPCS3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HeLa cells, MG-63 cells, U2OS cells, mouse kidney tissue, mouse liver tissue, rat kidney tissue Positive IHC detected in: human placenta tissue, rat liver tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information The microsomal signal peptidase complex (SPC), which is an essential membrane component in the endoplasmic reticulum (ER), has five subunits, including SEC11A, SEC11C, SPCS1, SPCS2, and SPCS3. Functionally, SPC removes signal peptides (SPs) from a series of secretory pre-proteins with high specificity. The dysregulation of the other SPC subunits was also observed in some tumors. SPCS3 expression can be used as a component of a risk score model to predict the survival of patients with pancreatic adenocarcinoma. (PMID: 35653344) Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag11780 Product name: Recombinant human SPCS3 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-180 aa of BC047290 Sequence: MNTVLSRANSLFAFSLSVMAALTFGCFITTAFKDRSVPVRLHVSRIMLKNVEDFTGPRERSDLGFITFDITADLENIFDWNVKQLFLYLSAEYSTKNNALNQVVLWDKIVLRGDNPKLLLKDMKTKYFFFDDGNGLKGNRNVTLTLSWNVVPNAGILPLVTGSGHVSVPFPDTYEITKSY Predict reactive species Full Name: signal peptidase complex subunit 3 homolog (S. cerevisiae) Calculated Molecular Weight: 180 aa, 20 kDa Observed Molecular Weight: 23 kDa GenBank Accession Number: BC047290 Gene Symbol: SPCS3 Gene ID (NCBI): 60559 RRID: AB_3669279 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P61009 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.