Proteintech, 17650-1-AP, PAAF1 Polyclonal antibody

Size: 20ul / 150ul The PAAF1 (17650-1-AP) by Proteintech is a Polyclonal antibody targeting PAAF1 in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse samples 17650-1-AP targets PAAF1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: Jurkat cells, HEK-293 cells, human brain tissue, mouse brain tissue Positive IHC detected in: mouse kidney tissue, human brain tissue, human kidney tissue, human testis tissue, mouse brain tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: U2OS cells Recommended dilution Western Blot (WB): WB : 1:1000-1:6000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information PAAF1(Proteasomal ATPase-associated factor 1) is also named as WDR71 and belongs to the WD repeat PAAF1/RPN14 family. It functions as a negative regulator of the proteasome by controlling the assembly/disassembly of the proteasome. PAAF1 has 3 isoforms produced by alternative splicing. Specification Tested Reactivity: human, mouse Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag11892 Product name: Recombinant human PAAF1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-375 aa of BC028628 Sequence: MLVPCFLYSLQNRKPSLYGSLTCQGIGLDGIPEVTASEGFTVNEINKKSIHISCPKENASSKFLAPYTTFSRIHTKSITCLDISSRGGLGVSSSTDGTMKIWQASNGELRRVLEGHVFDVNCCRFFPSGLVVLSGGMDAQLKIWSAEDASCVVTFKGHKGGILDTAIVDRGRNVVSASRDGTARLWDCGRSACLGVLADCGSSINGVAVGAADNSINLGSPEQMPSEREVGTEAKMLLLAREDKKLQCLGLQSRQLVFLFIGSDAFNCCTFLSGFLLLAGTQDGNIYQLDVRSPRAPVQVIHRSGAPVLSLLSVRDGFIASQGDGSCFIVQQDLDYVTELTGADCDPVYKVATWEKQIYTCCRDGLVRRYQLSDL Predict reactive species Full Name: proteasomal ATPase-associated factor 1 Calculated Molecular Weight: 375 aa, 40 kDa Observed Molecular Weight: 30 kDa GenBank Accession Number: BC028628 Gene Symbol: PAAF1 Gene ID (NCBI): 80227 RRID: AB_2878424 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9BRP4 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.