Proteintech, 17699-1-AP, ICAM3/CD50 Polyclonal antibody

Size: 20ul / 150ul The ICAM3/CD50 (17699-1-AP) by Proteintech is a Polyclonal antibody targeting ICAM3/CD50 in WB, IHC, IP, ELISA applications with reactivity to human samples 17699-1-AP targets ICAM3/CD50 in WB, IHC, IP, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: Jurkat cells, U-937 cells, Ramos cells Positive IP detected in: Jurkat cells Positive IHC detected in: human colon tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:2400 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:20-1:200 Background Information ICAM3 (Intercellular adhesion molecule 3) is also named as CD50, CDw50 and ICAM-R. ICAM3 is a member of the intercellular adhesion molecule (ICAM) family. ICAM3, being expressed constitutively on T cells and other leukocytes, is a potent signalling molecule and a major ligand in the initiation of T cell-mediated immune responses (PMID: 1448173, PMID: 11812993). ICAM3 is the natural ligand for both DC-SIGN and L-SIGN (PMID: 17570115). ICAM3 is abundantly and constitutively expressed in all leukocytes and is probably the most important ligand for LFA-1 in initiating immune responses, and ICAM3 is a typical type I transmembrane glycoprotein (PMID: 39991572). The promoter region of ICAM3 can be bound by co-transcription factors such as ETs and C/EBP, promoting macrophage maturation as well as monocyte extravasation (PMID: 22479382). ICAM3 is widely expressed not only in lymphocytes such as T and B cells but also in many endothelial cells and dendritic cells (PMID: 39991572). Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag11955 Product name: Recombinant human ICAM3 protein Source: e coli. -derived, T-HIS Tag: 6*His Domain: 200-547 aa of BC058903 Sequence: GLGLFVNTSAPRQLRTFVLPVTPPRLVAPRFLEVETSWPVDCTLDGLFPASEAQVYLALGDQMLNATVMNHGDTLTATATATARADQEGAREIVCNVTLGGERREARENLTVFSFLGPIVNLSEPTAHEGSTVTVSCMAGARVQVTLDGVPAAAPGQPAQLQLNATESDDGRSFFCSATLEVDGEFLHRNSSVQLRVLYGPKIDRATCPQHLKWKDKTRHVLQCQARGNPYPELRCLKEGSSREVPVGIPFFVNVTHNGTYQCQASSSRGKYTLVVVMDIEAGSSHFVPVFVAVLLTLGVVTIVLALMYVFREHQRSGSYHVREESTYLPLTSMQPTEAMGEEPSRAE Predict reactive species Full Name: intercellular adhesion molecule 3 Calculated Molecular Weight: 547 aa, 60 kDa Observed Molecular Weight: 110-160 kDa, 60 kDa GenBank Accession Number: BC058903 Gene Symbol: ICAM3/CD50 Gene ID (NCBI): 3385 RRID: AB_2248763 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P32942 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.