Proteintech, 17737-1-AP, EDC4 Polyclonal antibody

Size: 20ul / 150ul The EDC4 (17737-1-AP) by Proteintech is a Polyclonal antibody targeting EDC4 in WB, IHC, IF/ICC, ELISA applications with reactivity to human samples 17737-1-AP targets EDC4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HeLa cells, HepG2 cells Positive IHC detected in: human liver cancer tissue, human colon cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:1000-1:6000 Immunohistochemistry (IHC): IHC : 1:300-1:1200 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information Enhancer of mRNA-decapping protein 4 (EDC4) is a key regulator of mRNA decapping and degradation, playing a crucial role in the mRNA decay pathway. It is involved in the formation of processing bodies (P-bodies) in the cytoplasm, where it interacts with other decapping factors such as DCP1A and DCP2A to facilitate mRNA degradation (PMID: 16364915). EDC4 also interacts with the mammalian target of rapamycin complex 1 (mTORC1) and is involved in the regulation of immune system functions (PMID: 25514416; 25970328). Additionally, EDC4 has been shown to regulate DNA repair processes, with its deficiency leading to genome instability and hypersensitivity to DNA interstrand cross-linking drugs (PMID: 29511213). Specification Tested Reactivity: human Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag11784 Product name: Recombinant human EDC4 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-388 aa of BC064567 Sequence: MASCASIDIEDATQHLRDILKLDRPAGGPSAESPRPSSAYNGDLNGLLVPDPLCSGDSTSANKTGLRTMPPINLQEKQVICLSGDDSSTCIGILAKEVEIVASSDSSISSKARGSNKVKIQPVAKYDWEQKYYYGNLIAVSNSFLAYAIRAANNGSAMVRVISVSTSERTLLKGFTGSVADLAFAHLNSPQLACLDEAGNLFVWRLALVNGKIQEEILVHIRQPEGTPLNHFRRIIWCPFIPEESEDCCEESSPTVALLHEDRAEVWDLDMLRSSHSTWPVDVSQIKQGFIVVKGHSTCLSEGALSPDGTVLATASHDGYVKFWQIYIEGQDEPRCLHEWKPHDGRPLSCLLFCDNHKKQDPDVPFWRFLITGADQNRELKMWCTVSW Predict reactive species Full Name: enhancer of mRNA decapping 4 Calculated Molecular Weight: 1401 aa, 152 kDa Observed Molecular Weight: 160 kDa GenBank Accession Number: BC064567 Gene Symbol: EDC4 Gene ID (NCBI): 23644 RRID: AB_10665813 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q6P2E9 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.