Proteintech, 17790-1-AP, RIC8B Polyclonal antibody

Size: 20ul / 150ul The RIC8B (17790-1-AP) by Proteintech is a Polyclonal antibody targeting RIC8B in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse, rat samples 17790-1-AP targets RIC8B in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: fetal human brain tissue, mouse brain tissue, rat brain tissue Positive IHC detected in: human brain tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: MCF-7 cells Recommended dilution Western Blot (WB): WB : 1:2000-1:12000 Immunohistochemistry (IHC): IHC : 1:20-1:200 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information Ric8, also known as synembryn, is a guanine nucleotide exchange factor (GEF) that enhances variegated G protein signaling pathways, including Gα(q) and Gα(s) regulation of neurotransmission, Gα(i)-dependent mitotic spindle positioning during (asymmetric) cell division, and Gα(olf)-dependent odorant receptor signaling (PMID: 21467038). In mammals, two Ric8 homologues have been reported (Ric8A and Ric8B). Ric8B is able to potentiate G(s)/G(olf) signaling (PMID: 15829631; 16754875; 20133939). Specification Tested Reactivity: human, mouse, rat Cited Reactivity: mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag11888 Product name: Recombinant human RIC8B protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 145-495 aa of BC034220 Sequence: ELQGLPLLTQILESAFSIKWTDEYESAIDHNGPPLSPQETDCAIEALKALFNVTVDSWKVHKESDSHQFRVMAAVLRHCLLIVGPTEDKTEELHSNAVNLLSNVPVSCLDVLICPLTHEETAQEATTLDELPSNKTAEKETVLKNNTMVYNGMNMEAIHVLLNFMEKRIDKGSSYREGLTPVLSLLTECSRAHRNIRKFLKDQVLPPLRDVTNRPEVGSTVRNKLVRLMTHVDLGVKQIAAEFLFVLCKERVDSLLKYTGYGNAAGLLAARGLLAGGRGDNWYSEDEDTDTEEYKNAKPNINLITGHLEEPMPNPIDEMTEEQKEYEAMKLVNMLDKLSRRADVKDLHQDG Predict reactive species Full Name: resistance to inhibitors of cholinesterase 8 homolog B (C. elegans) Calculated Molecular Weight: 495 aa, 56 kDa Observed Molecular Weight: 70 kDa GenBank Accession Number: BC034220 Gene Symbol: RIC8B Gene ID (NCBI): 55188 RRID: AB_2285188 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9NVN3 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.