Proteintech, 17796-1-AP, ANT1/2 Polyclonal antibody

Size: 20ul / 150ul The ANT1/2 (17796-1-AP) by Proteintech is a Polyclonal antibody targeting ANT1/2 in WB, IHC, ELISA applications with reactivity to human, mouse, rat samples 17796-1-AP targets ANT1/2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: K-562 cells, HepG2 cells, mouse brain tissue, mouse heart tissue, mouse liver tissue, mouse kidney tissue, rat brain tissue, rat heart tissue, rat liver tissue Positive IHC detected in: human liver tissue, human liver cancer tissue, mouse heart tissue, mouse kidney tissue, human cervical cancer tissue, human stomach cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:1000-1:6000 Immunohistochemistry (IHC): IHC : 1:250-1:1000 Background Information Adenine nucleotide translocators (ANTs) are mitochondrial carrier proteins that exchange ATP and ADP between the cytoplasm and the mitochondrial matrix. Four isoforms of ANT were identified: ANT1 (encoded by SLC25A4) is highly expressed in different tissues; ANT2 (encoded by SLC25A5) is predominantly expressed in proliferating, regenerating, or undifferentiated cells; ANT3 is ubiquitously expressed; ANT4 is mainly expressed in testis and germ cells. This antibody recognizes both of ANT1 and ANT2. The predicted molecular weight of ANT1/2 protein is around 32 kDa. While 60 kDa protein can also be observed, which has been proposed as the dimer of ANT1/2. (22423112) Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag11956 Product name: Recombinant human SLC25A5 protein Source: e coli. -derived, T-HIS Tag: 6*His Domain: 1-298 aa of BC056160 Sequence: MTDAAVSFAKDFLAGGVAAAISKTAVAPIERVKLLLQVQHASKQITADKQYKGIIDCVVRIPKEQGVLSFWRGNLANVIRYFPTQALNFAFKDKYKQIFLGGVDKRTQFWRYFAGNLASGGAAGATSLCFVYPLDFARTRLAADVGKAGAEREFRGLGDCLVKIYKSDGIKGLYQGFNVSVQGIIIYRAAYFGIYDTAKGMLPDPKNTHIVISWMIAQTVTAVAGLTSYPFDTVRRRMMMQSGRKGTDIMYTGTLDCWRKIARDEGGKAFFKGAWSNVLRGMGGAFVLVLYDEIKKYT Predict reactive species Full Name: solute carrier family 25 (mitochondrial carrier; adenine nucleotide translocator), member 5 Calculated Molecular Weight: 298 aa, 33 kDa Observed Molecular Weight: 30-32 kDa, 60 kDa GenBank Accession Number: BC056160 Gene Symbol: SLC25A5 Gene ID (NCBI): 292 RRID: AB_2190358 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P05141 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.