Proteintech, 17937-1-AP, PTPRR Polyclonal antibody

Size: 20ul / 150ul The PTPRR (17937-1-AP) by Proteintech is a Polyclonal antibody targeting PTPRR in WB, ELISA applications with reactivity to human, mouse, rat samples 17937-1-AP targets PTPRR in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HeLa cells, mouse brain tissue, COLO 320 cells, SH-SY5Y cells Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Background Information PTPRR(protein tyrosine phosphatase, receptor type, R) is also named as PCPTP1, PTP-SL, PTPBR7 and belongs to the protein-tyrosine phosphatase family. It sequesters mitogen-activated protein kinases (MAPKs) such as MAPK1, MAPK3 and MAPK14 in the cytoplasm in an inactive form. It has four isoforms that they have molecular weights of 72, 60, 42 and 37 kDa, respectively, and differ in the length of their N-terminal part which result in distinct subcellular localizations. All PTPRR isoforms are found to be short-lived proteins, with half-lives of around 3-5 hours, and are constitutively phosphorylated on one or two of the previously identified protein kinase A and MAP kinase target sites. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag12145 Product name: Recombinant human PTPRR protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-412 aa of BC110900 Sequence: MILYRLKERFQLSLRQDKEKNQEIHLSPITLQPALSEAKTVHSMVQPEQAPKVLNVVVDPQGRGAPEIRATTATSVCPSPFKMKPIGLQERRGSNVSLTLDMSSLGNIEPFVSIPTPREKVAMEYLQSASRILTRSQLRDVVASSHLLQSEFMEIPMNFVDPKEIDIPRHGTKNRYKTILPNPLSRVCLRPKNVTDSLSTYINANYIRGYSGKEKAFIATQGPMINTVDDFWQMVWQEDSPVIVMITKLKEKNEKCVLYWPEKRGIYGKVEVLVISVNECDNYTIRNLVLKQGSHTQHVKHYWYTSWPDHKTPDSAQPLLQLMLDVEEDRLASQGRGPVVVHCSAGIGRTGCFIATSIGCQQLKEEGVVDALSIVCQLRMDRGGMVQTSEQYEFVHHALCLYESRLSAETVQ Predict reactive species Full Name: protein tyrosine phosphatase, receptor type, R Calculated Molecular Weight: 657 aa, 74 kDa Observed Molecular Weight: 32 kDa, 47 kDa, 65 kDa GenBank Accession Number: BC110900 Gene Symbol: PTPRR Gene ID (NCBI): 5801 RRID: AB_10694430 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q15256 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.