Proteintech, 17948-1-AP, PRR5 Polyclonal antibody

Size: 20ul / 150ul The PRR5 (17948-1-AP) by Proteintech is a Polyclonal antibody targeting PRR5 in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human, mouse, rat samples 17948-1-AP targets PRR5 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: human kidney tissue, COLO 320 cells, HEK-293 cells, HepG2 cells, human brain tissue, mouse brain tissue Positive IP detected in: mouse brain tissue Positive IHC detected in: mouse testis tissue, mouse brain tissue, mouse kidney tissue, human kidney tissue, human placenta tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:500-1:2400 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100 Background Information PRR5, also named as PROTOR1 and PP610, is a subunit of mTORC2, which regulates cell growth and survival in response to hormonal signals. PRR5 plays an important role in regulation of PDGFRB expression and in modulation of platelet-derived growth factor signaling. It acts as a tumor suppressor in breast cancer. PRR5 has some isoforms with MW 31-33 kDa and 41-50 kDa. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag12424 Product name: Recombinant human PRR5 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-287 aa of BC016921 Sequence: MAPMPTGQKLLDSLAETWDFFFSDVLPMLQAIFYPVQGKEPSVRQLALLHFRNAITLSVKLEDALARAHARVPPAIVQMLLVLQGVHESRGVTEDYLRLETLVQKVVSPYLGTYGLHSSEGPFTHSCILEKRLLRRSRSGDVLAKNPVVRSKSYNTPLLNPVQEHEAEGAAAGGTSIRRHSVSEMTSCPEPQGFSDPPGQGPTGTFRSSPAPHSGPCPSRLYPTTQPPEQGLDPTRSSLPRSSPENLVDQILESVDSDSEGIFIDFGRGRGSGMSDLEGSGGRQSVV Predict reactive species Full Name: proline rich 5 (renal) Calculated Molecular Weight: 31 kDa, 43 kDa Observed Molecular Weight: 33 kDa, 50 kDa GenBank Accession Number: BC016921 Gene Symbol: PRR5 Gene ID (NCBI): 55615 RRID: AB_2268865 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P85299 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.