Proteintech, 17954-1-AP, HFE Polyclonal antibody

Size: 20ul / 150ul The HFE (17954-1-AP) by Proteintech is a Polyclonal antibody targeting HFE in WB, ELISA applications with reactivity to human samples 17954-1-AP targets HFE in WB, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: A549 cells, A375 cells, Caco-2 cells, HeLa cells, HepG2 cells, human placenta tissue Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Background Information HFE is an important iron metabolism regulatory protein, which regulates the production of hepcidin through interactions with other proteins such as transferrin receptor 1 (TfR1) and TfR2. HFE is the most common pathogenic gene for hereditary hemochromatosis, which is a hereditary disease of iron accumulation in multiple organs caused by mutations in genes regulating iron metabolism. Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag12448 Product name: Recombinant human HFE protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 29-295 aa of BC074721 Sequence: SLHYLFMGASEQDLGLSLFEALGYVDDQLFVFYDHESRRVEPRTPWVSSRISSQMWLQLSQSLKGWDHMFTVDFWTIMENHNHSKESHTLQVILGCEMQEDNSTEGYWKYGYDGQDHLEFCPDTLDWRAAEPRAWPTKLEWERHKIRARQNRAYLERDCPAQLQQLLELGRGVLDQQEGSESSSLPGKVTTLRCRALNYYPQNITMKWLKDKQPMDAKEFEPKDVLPNGDGTYQGWITLAVPPGEEQRYTCQVEHPGLDQPLIVIWE Predict reactive species Full Name: hemochromatosis Calculated Molecular Weight: 345 aa, 40 kDa Observed Molecular Weight: 45-58 kDa GenBank Accession Number: BC074721 Gene Symbol: HFE Gene ID (NCBI): 3077 RRID: AB_10596779 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q30201 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.