Proteintech, 18166-1-AP, WISP-1/CCN4 Polyclonal antibody

Size: 20ul / 150ul The WISP-1/CCN4 (18166-1-AP) by Proteintech is a Polyclonal antibody targeting WISP-1/CCN4 in WB, IHC, ELISA applications with reactivity to human, mouse samples 18166-1-AP targets WISP-1/CCN4 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: COLO 320 cells, HEK-293 cells, mouse testis tissue, mouse liver tissue Positive IHC detected in: human stomach cancer tissue, human small intestine tissue, human lung cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information WISP1( Wnt-induced secreted protein 1), also known as CCN4, is a secreted, matricellular protein allocated to the CCN protein family. The CCN protein family consists of six, modular structured, secreted proteins ( Cyr61, CTGF, Nov, WISP1, WISP2, WISP3 ). WISP1 may be downstream in the WNT1 signaling pathway that is relevant to malignant transformation. WISP1 is expressed at a high level in fibroblast cells, and overexpressed in colon tumors. Its expression is associated with proliferation, cytoprotection, as well as extracellular matrix production. 18166-1-AP antibody detects the 40-45 kDa protein in SDS-PAGE. Specification Tested Reactivity: human, mouse Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag12657 Product name: Recombinant human WISP1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 132-367 aa of BC074841 Sequence: NGQSFQPNCKYNCTCIDGAVGCTPLCLRVRPPRLWCPHPRRVSIPGHCCEQWVCEDDAKRPRKTAPRDTGAFDAVGEVEAWHRNCIAYTSPWSPCSTSCGLGVSTRISNVNAQCWPEQESRLCNLRPCDVDIHTLIKAGKKCLAVYQPEASMNFTLAGCISTRSYQPKYCGVCMDNRCCIPYKSKTIDVSFQCPDGLGFSRQVLWINACFCNLSCRNPNDIFADLESYPDFSEIAN Predict reactive species Full Name: WNT1 inducible signaling pathway protein 1 Calculated Molecular Weight: 367 aa, 40 kDa Observed Molecular Weight: 40-45 kDa GenBank Accession Number: BC074841 Gene Symbol: WISP1 Gene ID (NCBI): 8840 RRID: AB_10805764 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: O95388 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.