Proteintech, 18182-1-AP, SLC28A3 Polyclonal antibody

Size: 20ul / 150ul The SLC28A3 (18182-1-AP) by Proteintech is a Polyclonal antibody targeting SLC28A3 in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse samples 18182-1-AP targets SLC28A3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: HL-60 cells, mouse kidney tissue, mouse liver tissue Positive IHC detected in: human pancreas cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunohistochemistry (IHC): IHC : 1:400-1:1600 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information SLC28A3 (Solute carrier family 28 member 3), also named CNT3, has sodium-dependent, pyrimidine- and purine-selective (PMID: 11032837, PMID: 15861042, PMID: 16446384, PMID: 17140564, PMID: 21998139). It is involved in the homeostasis of endogenous nucleosides (PMID: 11032837, PMID: 15861042). SLC28A3 is able to transports uridine, gemcitabine, 3'-azido-3'-deoxythymidine (AZT), ribavirin and 3-deazauridine (PMID: 21795683, PMID: 11032837, PMID: 17140564). SLC28A3, the human concentrative nucleoside transporter, plays a role in mediating the cellular entry of a broad array of physiological nucleosides and synthetic anticancer nucleoside analog drugs (PMID: 15738947). Variants in SLC28A3 and UGT1A6 act as genetic markers predictive of anthracycline-induced cardiotoxicity (PMID: 23441093). Specification Tested Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag12887 Product name: Recombinant human SLC28A3 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-186 aa of BC093821 Sequence: MELRSTAAPRAEGYSNVGFQNEENFLENENTSGNNSIRSRAVQSREHTNTKQDEEQVTVEQDSPRNREHMEDDDEEMQQKGCLERRYDTVCGFCRKHKTTLRHIIWGILLAGYLVMVISACVLNFHRALPLFVITVAAIFFVVWDHLMAKYEHRIDEMLSPGRRLLNSHWFWLKWVIWSSLVLAVI Predict reactive species Full Name: solute carrier family 28 (sodium-coupled nucleoside transporter), member 3 Calculated Molecular Weight: 691 aa, 77 kDa Observed Molecular Weight: 70-75 kDa GenBank Accession Number: BC093821 Gene Symbol: SLC28A3 Gene ID (NCBI): 64078 RRID: AB_3085558 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9HAS3 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.