Proteintech, 18352-1-AP, KLF13 Polyclonal antibody

Size: 20ul / 150ul The KLF13 (18352-1-AP) by Proteintech is a Polyclonal antibody targeting KLF13 in WB, IHC, ELISA applications with reactivity to human, rat, mouse samples 18352-1-AP targets KLF13 in WB, IHC, IF, ELISA applications and shows reactivity with human, rat, mouse samples. Tested Applications Positive WB detected in: HT-29 cells, rat liver tissue Positive IHC detected in: human brain tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Immunohistochemistry (IHC): IHC : 1:20-1:200 Background Information Kruppel-like factor 13(KLF13) is a member of transcription actors, which contains 3 zinc finger DNA-binding domains. Theses transcription factors bind to CG-rich sequences and related GT and CACCC boxes. KLF13 involved in activation of transcription of variety of genes in the cells of erythroid lineage, such as GATA1, glycophorin B. It also binds to the A and A/B RANTES promoter binding sites, the same as NFKB, and then transactivates the RANTES gene. The calculated molecular weight of KLF13 is 31 kDa, but the modified KLF13 protein is about 37-45 kDa. Specification Tested Reactivity: human, rat, mouse Cited Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag13200 Product name: Recombinant human KLF13 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-288 aa of BC012741 Sequence: MAAAAYVDHFAAECLVSMSSRAVVHGPREGPESRPEGAAVAATPTLPRVEERRDGKDSASLFVVARILADLNQQAPAPAPAERREGAAARKARTPCRLPPPAPEPTSPGAEGAAAAPPSPAWSEPEPEAGLEPEREPGPAGSGEPGLRQRVRRGRSRADLESPQRKHKCHYAGCEKVYGKSSHLKAHLRTHTGERPFACSWQDCNKKFARSDELARHYRTHTGEKKFSCPICEKRFMRSDHLTKHARRHANFHPGMLQRRGGGSRTGSLSDYSRSDASSPTISPASSP Predict reactive species Full Name: Kruppel-like factor 13 Calculated Molecular Weight: 31 kDa Observed Molecular Weight: 37-45 kDa GenBank Accession Number: BC012741 Gene Symbol: KLF13 Gene ID (NCBI): 51621 RRID: AB_2132399 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q9Y2Y9 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.