Proteintech, 18375-1-AP, KISS1 Polyclonal antibody

Size: 20ul / 150ul The KISS1 (18375-1-AP) by Proteintech is a Polyclonal antibody targeting KISS1 in WB, IHC, ELISA applications with reactivity to human samples 18375-1-AP targets KISS1 in WB, IHC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: human placenta tissue Positive IHC detected in: human placenta tissue, human liver cancer tissue, human lung cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Immunohistochemistry (IHC): IHC : 1:400-1:1600 Background Information KISS1, also known as kisspeptin, is a metastasis suppressor protein that suppresses metastases of human melanomas and breast carcinomas without affecting tumorigenicity. It may regulate events downstream of cell-matrix adhesion, perhaps involving cytoskeletal reorganization. A C-terminally amidated peptide of KISS1, metastin (kisspeptin-54 or kp-54), functions as the endogenous ligand of the G-protein coupled receptor GPR54 (PMID: 11457843; 11385580). KISS1-GPR54 signaling is involved in the regulation of reproduction, playing an important role in initiating secretion of gonadotropin-releasing hormone (GnRH) at puberty (PMID: 18753370). Specification Tested Reactivity: human Cited Reactivity: human, rat Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag13240 Product name: Recombinant human KISS1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-138 aa of BC022819 Sequence: MNSLVSWQLLLFLCATHFGEPLEKVASVGNSRPTGQQLESLGLLAPGEQSLPCTERKPAATARLSRRGTSLSPPPESSGSPQQPGLSAPHSRQIPAPQGAVLVQREKDLPNYNWNSFGLRFGKREAAPGNHGRSAGRG Predict reactive species Full Name: KiSS-1 metastasis-suppressor Calculated Molecular Weight: 15 kDa Observed Molecular Weight: 15 kDa GenBank Accession Number: BC022819 Gene Symbol: KISS1 Gene ID (NCBI): 3814 RRID: AB_11182705 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q15726 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.