Proteintech, 18413-1-AP, FURIN Polyclonal antibody

Size: 20ul / 150ul The FURIN (18413-1-AP) by Proteintech is a Polyclonal antibody targeting FURIN in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human samples 18413-1-AP targets FURIN in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HepG2 cells, COLO 320 cells, HeLa cells Positive IP detected in: HeLa cells Positive IHC detected in: human placenta tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:20-1:200 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information FURIN, also named FUR, PACE, PCSK3, SPC1, and Kex2p, is likely to represent the ubiquitous endoprotease activity within constitutive secretory pathways and is capable of cleavage at the RX(K/R)R consensus motif. Furin is synthesized as an inactive zymogen that may minimize the occurrence of premature enzymatic activity that would lead to alternative protein activation or degradation. The molecular weight of mature furin is 75-90 kDa and that of pre-pro furin is 95 kDa. With glycosylation and other post-translational modifications, the molecular weight of pre-pro furin will be migrated to 100-110 kDa. (PMID: 1644796, 1885622, 31336100, 35322530). Specification Tested Reactivity: human Cited Reactivity: human, mouse, rat, chicken Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag13261 Product name: Recombinant human FURIN protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 495-794 aa of BC012181 Sequence: YNRRGDLAIHLVSPMGTRSTLLAARPHDYSADGFNDWAFMTTHSWDEDPSGEWVLEIENTSEANNYGTLTKFTLVLYGTAPEGLPVPPESSGCKTLTSSQACVVCEEGFSLHQKSCVQHCPPGFAPQVLDTHYSTENDVETIRASVCAPCHASCATCQGPALTDCLSCPSHASLDPVEQTCSRQSQSSRESPPQQQPPRLPPEVEAGQRLRAGLLPSHLPEVVAGLSCAFIVLVFVTVFLVLQLRSGFSFRGVKVYTMDRGLISYKGLPPEAWQEECPSDSEEDEGRGERTAFIKDQSAL Predict reactive species Full Name: furin (paired basic amino acid cleaving enzyme) Calculated Molecular Weight: 87 kDa Observed Molecular Weight: 90-110 kDa GenBank Accession Number: BC012181 Gene Symbol: FURIN Gene ID (NCBI): 5045 RRID: AB_2878542 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P09958 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.