Proteintech, 18501-1-AP, HOXA9 Polyclonal antibody

Size: 20ul / 150ul The HOXA9 (18501-1-AP) by Proteintech is a Polyclonal antibody targeting HOXA9 in WB, IHC, IP, ELISA applications with reactivity to human, mouse samples 18501-1-AP targets HOXA9 in WB, IHC, IP, chIP, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: EC109 cells, A431 cells, HaCaT cells Positive IP detected in: mouse appendix tissue Positive IHC detected in: mouse skin tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:500-1:2000 Background Information HOXA9, also known as HOX1G, belongs to the Abd-B homeobox family and transiently interacts with PRMT5 in TNF-alpha stimulated endothelial cells. HOXA9 is methylated on Arg-140 by PRMT5and methylation is critical for E-selectin induction. HOXA9 as a sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis. HOXA9 is required for induction of E-selectin and VCAM-1, on the endothelial cells surface at sites of inflammation. HOXA9 is 30 kDa, but methylation of HOXA9 is about 35-40 kDa. (PMID: 10082572) Specification Tested Reactivity: human, mouse Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag13350 Product name: Recombinant human HOXA9 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-272 aa of BC010023 Sequence: MATTGALGNYYVDSFLLGADAADELSVGRYAPGTLGQPPRQAATLAEHPDFSPCSFQSKATVFGASWNPVHAAGANAVPAAVYHHHHHHPYVHPQAPVAAAAPDGRYMRSWLEPTPGALSFAGLPSSRPYGIKPEPLSARRGDCPTLDTHTLSLTDYACGSPPVDREKQPSEGAFSENNAENESGGDKPPIDPNNPAANWLHARSTRKKRCPYTKHQTLELEKEFLFNMYLTRDRRYEVARLLNLTERQVKIWFQNRRMKMKKINKDRAKDE Predict reactive species Full Name: homeobox A9 Calculated Molecular Weight: 30 kDa Observed Molecular Weight: 40 kDa GenBank Accession Number: BC010023 Gene Symbol: HOXA9 Gene ID (NCBI): 3205 RRID: AB_2861187 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P31269 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.