Proteintech, 18543-1-AP, PDLIM4 Polyclonal antibody

Size: 20ul / 150ul The PDLIM4 (18543-1-AP) by Proteintech is a Polyclonal antibody targeting PDLIM4 in WB, IHC, IF/ICC, ELISA applications with reactivity to human samples 18543-1-AP targets PDLIM4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: A431 cells, K-562 cells Positive IHC detected in: human skin cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: U2OS cells Recommended dilution Western Blot (WB): WB : 1:1000-1:6000 Immunohistochemistry (IHC): IHC : 1:300-1:1200 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information PDLIM4, also known as RIL, is a member of the ALP/Enigma family. PDLIM4 contains one PDZ(DHR) domain and one LIM zinc-binding domain. The LIM zinc-binding domain interacts with the second and fourth PDZ domains of Fap-1, possibly resulting in the dephosphorylation of PDLIM4 (PMID: 21636573). PDLIM4 is involved in osteoblast development and function. The variation of the PDLIM4 locus may be an important determinant of osteoporosis (PMID: 12908099). Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag13424 Product name: Recombinant human PDLIM4 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-330 aa of BC016765 Sequence: MPHSVTLRGPSPWGFRLVGGRDFSAPLTISRVHAGSKAALAALCPGDLIQAINGESTELMTHLEAQNRIKGCHDHLTLSVSRPEGRSWPSAPDDSKAQAHRIHIDPEIQDGSPTTSRGPSGTGTGPEDGRPSLGSPYGQPPRFPVPHNGSSEATLPAQMSTLHVSPPPSADPARGLPRSRDCRVDLGSEVYRMLREPAEPVAAEPKQSGSFRYLQGMLEAGEGGDWPGPGGPRNLKPTASKLGAPLSGLQGLPECTRCGHGIVGTIVKARDKLYHPECFMCSDCGLNLKQRGYFFLDERLYCESHAKARVKPPEGYDVVAVYPNAKVELV Predict reactive species Full Name: PDZ and LIM domain 4 Calculated Molecular Weight: 35 kDa Observed Molecular Weight: 35 kDa GenBank Accession Number: BC016765 Gene Symbol: PDLIM4 Gene ID (NCBI): 8572 RRID: AB_3085565 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P50479 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.