Proteintech, 18703-1-AP, CPS1 Polyclonal antibody

Size: 20ul / 150ul The CPS1 (18703-1-AP) by Proteintech is a Polyclonal antibody targeting CPS1 in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse, rat samples 18703-1-AP targets CPS1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: mouse liver tissue, HeLa cells, rat liver tissue Positive IHC detected in: human liver cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:2000-1:14000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information CPS1 is an enzyme that catalyzes the first committed step of the hepatic urea cycle, which is important in the removal of excess urea from cells. Expressed primarily in the liver and small intestine, CPS1 can be used as a marker for mitochondria in these tissues. In addition, CPS1 expression appears to be lost in adenocarcinomas of the small intestine. The antibody can recognize two isoforms of this gene around 165 kDa and 116 kDa. Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: carbamoyl-phosphate synthetase 1, mitochondrial Calculated Molecular Weight: 165 kDa Observed Molecular Weight: 140-165 kDa GenBank Accession Number: BC140943 Gene Symbol: CPS1 Gene ID (NCBI): 1373 RRID: AB_2084238 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: P31327 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.