Proteintech, 18834-1-AP, ARHGAP24 Polyclonal antibody

Size: 20ul / 150ul The ARHGAP24 (18834-1-AP) by Proteintech is a Polyclonal antibody targeting ARHGAP24 in IHC, ELISA applications with reactivity to human, mouse, rat, Canine samples 18834-1-AP targets ARHGAP24 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat, Canine samples. Tested Applications Positive IHC detected in: human kidney tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Immunohistochemistry (IHC): IHC : 1:20-1:200 Background Information ARHGAP24, also named as FILGAP, is involved in cell polarity, cell morphology and cytoskeletal organization. ARHGAP24 acts as a GTPase activator for the Rac-type GTPase by converting it to an inactive GDP-bound state. ARHGAP24 controls actin remodeling by inactivating Rac downstream of Rho leading to suppress leading edge protrusion and promotes cell retraction to achieve cellular polarity. ARHGAP24 can suppress the activity of RAC1 and CDC42 in vitro. Its overexpression induces cell rounding with partial or complete disruption of actin stress fibers and formation of membrane ruffles, lamellipodia, and filopodia. Isoform 2 is a vascular cell-specific GAP involved in modulation of angiogenesis. The antibody may recognize all the isoforms of ARHGAP24 except isoform 5. Specification Tested Reactivity: human, mouse, rat, Canine Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: Peptide Predict reactive species Full Name: Rho GTPase activating protein 24 Calculated Molecular Weight: 84 kDa Observed Molecular Weight: 73 kDa GenBank Accession Number: BC098580 Gene Symbol: ARHGAP24 Gene ID (NCBI): 83478 RRID: AB_10603354 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q8N264 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.