Proteintech, 18884-1-AP, Glutaredoxin 5 Polyclonal antibody

Size: 20ul / 150ul The Glutaredoxin 5 (18884-1-AP) by Proteintech is a Polyclonal antibody targeting Glutaredoxin 5 in WB, IF/ICC, ELISA applications with reactivity to human, mouse samples 18884-1-AP targets Glutaredoxin 5 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: HepG2 cells, MCF-7 cells, mouse heart tissue Positive IF/ICC detected in: MCF-7 cells Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information Glutaredoxin 5 (GLRX5) is an essential protein engaging in mitochondrial iron-sulfur cluster (ISC) transfers to iron regulatory protein (IRP), m-aconitase, and ferrochelatase. GLRX5 deficiency impairs heme biosynthesis, causing sideroblastic anemia and variant nonketotic hyperglycinemia in humans. Mutations of GLRX5 lead to distinct effects on downstream ISC biosynthesis and maturation. Specification Tested Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag13445 Product name: Recombinant human GLRX5 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-157 aa of BC023528 Sequence: MSGSLGRAAAALLRWGRGAGGGGLWGPGVRAAGSGAGGGGSAEQLDALVKKDKVVVFLKGTPEQPQCGFSNAVVQILRLHGVRDYAAYNVLDDPELRQGIKDYSNWPTIPQVYLNGEFVGGCDILLQMHQNGDLVEELKKLGIHSTLLDEKKDQDSK Predict reactive species Full Name: glutaredoxin 5 Calculated Molecular Weight: 157 aa, 17 kDa Observed Molecular Weight: 10-15 kDa GenBank Accession Number: BC023528 Gene Symbol: GLRX5 Gene ID (NCBI): 51218 RRID: AB_3669306 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q86SX6 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.