Proteintech, 18892-1-AP, PTRF Polyclonal antibody

Size: 20ul / 150ul The PTRF (18892-1-AP) by Proteintech is a Polyclonal antibody targeting PTRF in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human, mouse, rat samples 18892-1-AP targets PTRF in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HT-1080 cells, rat heart tissue, mouse heart tissue, mouse lung tissue Positive IP detected in: HT-1080 cells Positive IHC detected in: human normal colon, mouse brown adipose tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:500-1:2000 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information Polymerase I and transcript release factor(PTRF) is an essential factor in the biogenesis of caveolae, which involve in numerous process, such as signal transduction and membrane and lipid trafficking. PTRF can terminate transciption of RNA Pol I, which involves pausing of transcription by TTF1, and dissocaition of the transcription complex, release the complex from the template. The calculated molecular weight of PTRF is 43 kDa, but modified PTRF is about 50-55kDa. (PMID: 11139612 ) Specification Tested Reactivity: human, mouse, rat Cited Reactivity: human, mouse, monkey, zebrafish Host / Isotype: Rabbit / IgG Class: Polyclonal Type: Antibody Immunogen: CatNo: Ag13486 Product name: Recombinant human PTRF protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-198 aa of BC008849 Sequence: MEDAAALELSSDEAVEVEEVIEESRAERIKRSGLRRVDDFKKAFSKEKMEKTKVRTRENLEKTRLKTKENLEKTRHTLEKRMNKLGTRLVPAERREKLKTSRDKLRKSFTPDHVVYARSKTAVYKVPPFTFHVKKIREGQVEVLKATEMVEVGADDDEGGAERGEAGDLRRGSSPDVHALLEITEESDAVLVDKSDSD Predict reactive species Full Name: polymerase I and transcript release factor Calculated Molecular Weight: 43 kDa Observed Molecular Weight: 50-55 kDa GenBank Accession Number: BC008849 Gene Symbol: PTRF Gene ID (NCBI): 284119 RRID: AB_10596795 Conjugate: Unconjugated Form: Liquid Purification Method: Antigen affinity purification UNIPROT ID: Q6NZI2 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.